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559-pET28b-Nhe1 - Revision history
2026-10-18T14:13:11Z
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Paul.xu at 18:08, 10 May 2013
2013-05-10T18:08:20Z
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<p><b>New page</b></p><div>== '''Cloning of 559-pET28b-Nhe1''' ==<br />
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'''Purpose:''' Cloning of 559-pET28b-Nhe1 <br />
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'''Date: '''2013-5-3--2013-5-7 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:559''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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== Colony Screening ==<br />
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Date: 2013-5-7 <br />
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Make miniprep and digest Xho1 + Nde1 <br />
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[[Image:559 digest 5-7-2013.JPG]]<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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*&amp;amp;lt;br&amp;amp;gt;<br />
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== '''Conclusion''' ==<br />
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== '''fix of Cloning of 559-pET28b-Nhe1''' ==<br />
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'''Purpose:''' fix of Cloning of 1519-pT7A3-T3-DNA-Ligase <br />
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'''Date: '''2013-5-6--2013-5-10 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:559''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:559pcr1+pcr2_5-7-2013.JPG]]<br> <br />
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[[Image:559PCR_5-7-2013.JPG]]<br />
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[[Image:553+559+1724+1741+1745clone_5-8-2013.JPG]]<br />
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<br> <br />
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<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-5-10 <br />
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Make miniprep and digest with&nbsp; Nde1 + Xho1 <br />
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[[Image:559 digest 5-10-2013.JPG]]<br> <br />
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<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu