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562 pEC-EcoR1 - Revision history
2026-10-18T14:09:46Z
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Paul.xu at 18:01, 20 May 2013
2013-05-20T18:01:35Z
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<p><b>New page</b></p><div>== '''Cloning of 562 pEC-EcoR1''' ==<br />
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'''Purpose:''' Cloning of 562 pEC-EcoR1 <br />
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'''Date: '''2013-4--- <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:562''' <br />
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'''Experimenters:''' Shanyi <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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gene synthesis <br> <br />
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[[Image:562firstPCR.JPG]]<br> <br />
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join two fragments by PCR <br />
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[[Image:562PCR.JPG]]<br> <br />
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<br> digest with Nde1+Xho1 <br> <br />
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[[Image:Digest for 562 563.JPG]]<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-4- <br />
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Make miniprep and digest with Nde1+Xho1 <br />
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[[Image:560 561 562digest.JPG]]<br> <br> <br />
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=== cloning ===<br />
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FIX ONE GAP,USE 562-6 as template,use EcoRI-1-1and EcoRI-1-6, EcoRI-1-7 and EcoRI-2-12 as primers<br> <br />
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[[Image:561redo 562fixpcr.JPG]] <br />
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join two fragments by PCR <br />
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[[Image:562fix 11443rdfixpcr.JPG]]<br> digest with Nde1+Xho1 <br> <br />
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[[Image:DIGEST FOR562fix 11443rdfixpcr.JPG]]<br> <br />
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<br> <br />
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<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-4- <br />
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Make miniprep and digest with Nde1+Xho1 <br />
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[[Image:562fix_622_1144fix3_1740digest.JPG]]<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu