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622 pT7A3-8H-IL-2 - Revision history
2026-10-18T21:17:51Z
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Paul.xu at 15:35, 6 August 2013
2013-08-06T15:35:18Z
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<p><b>New page</b></p><div>== '''Cloning of 622 pT7A3-8H-IL-2''' ==<br />
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'''Purpose:''' Cloning of 622 pT7A3-8H-IL-2 <br />
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'''Date: '''2013-4--- <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:622''' <br />
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'''Experimenters:''' Shanyi <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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gene synthesis <br> <br />
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[[Image:622 623PCR Q5.JPG]]<br> <br />
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digest with Nde1+Xho1 <br> <br />
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[[Image:Digest for 622 623 .JPG]] <br />
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=== Colony Screening ===<br />
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Date: 2013-4-26 <br />
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Make miniprep and digest with Nde1+Xho1 <br />
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[[Image:560fix 561fix 622digest.JPG]]<br> <br />
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send 622-6 to sequence, IL-2 is right,but the vector is not pT7A3-8H,need to do subclone.<br> <br />
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=== cloning ===<br />
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digest with Nde1+Xho1 <br />
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[[Image:Digest_for622.JPG]]<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-4-26 <br />
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Make miniprep and digest with Nde1+Xho1 <br />
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[[Image:560_642_622_641digest.JPG]]<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu