https://eln.excellgen.com/index.php?action=history&feed=atom&title=626_1717_1719_1720 626 1717 1719 1720 - Revision history 2026-10-18T02:17:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=626_1717_1719_1720&diff=9071&oldid=prev Paul.xu at 15:28, 6 May 2013 2013-05-06T15:28:09Z <p></p> <p><b>New page</b></p><div>= &lt;br&gt;&#039;&#039;&#039;2013/4/5&#039;&#039;&#039;&lt;br&gt; =<br /> <br /> [[Image:626+1717+1719+1720 protein screening 5-2-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; width=&quot;200&quot; border=&quot;1&quot;<br /> |-<br /> | align=&quot;center&quot; style=&quot;background:#f0f0f0;&quot; | &#039;&#039;&#039;lane&#039;&#039;&#039; <br /> | align=&quot;center&quot; style=&quot;background:#f0f0f0;&quot; | &#039;&#039;&#039;clone ID&#039;&#039;&#039; <br /> | align=&quot;center&quot; style=&quot;background:#f0f0f0;&quot; | &#039;&#039;&#039;host&#039;&#039;&#039; <br /> | align=&quot;center&quot; style=&quot;background:#f0f0f0;&quot; | &#039;&#039;&#039;induction&#039;&#039;&#039; <br /> | align=&quot;center&quot; style=&quot;background:#f0f0f0;&quot; | &#039;&#039;&#039;purification&#039;&#039;&#039; <br /> | align=&quot;center&quot; style=&quot;background:#f0f0f0;&quot; | &#039;&#039;&#039;MW&#039;&#039;&#039;<br /> |-<br /> | 1<br /> | 626,pT7A3-Taq_d289 <br /> | Nico21DE3plysN<br /> | 0.2 mM IPTG 25 oC o/n<br /> | Ni beads<br /> | 61KD/5.97<br /> |-<br /> | 2<br /> | 1717, pT7A3-6H-(TEV)-MBP-(3C) <br /> | Nico21DE3plysN<br /> | 0.2 mM IPTG 25 oC o/n<br /> | Ni beads<br /> | 49KD/5.46<br /> |-<br /> | 3<br /> | 1719, pT7A3-MBP-(E3C)-NRF2<br /> | BL21DE3pRare2pLysS<br /> | 0.2 mM IPTG 25 oC o/n<br /> | Ni beads<br /> | 112KD/4.82<br /> |-<br /> | 4<br /> | 1720, pT7A3-MBP-(E3C)-Keap1<br /> | BL21DE3pRare2pLysS<br /> | 0.2 mM IPTG 25 oC o/n<br /> | Ni beads<br /> | 115KD/5.57<br /> |-<br /> | 5<br /> | 1719, pT7A3-MBP-(E3C)-NRF2, fraction 38<br /> | BL21DE3pRare2pLysS<br /> | 0.2 mM IPTG 25 oC o/n<br /> | 5ml HisTrap HP<br /> | 112KD/4.82<br /> |-<br /> | 6<br /> | 1719, pT7A3-MBP-(E3C)-NRF2, fraction 23<br /> | BL21DE3pRare2pLysS<br /> | 0.2 mM IPTG 25 oC o/n<br /> | 5ml HisTrap HP<br /> | 112KD/4.82<br /> |-<br /> | 7<br /> | Cre, 3.9ug/ul<br /> | __<br /> | __<br /> | __<br /> | __<br /> |-<br /> | 8<br /> | cre,4/2/2013<br /> | __<br /> | __<br /> | __<br /> | __<br /> |-<br /> | 9<br /> | 1624,pCold-6His-GST-(E)-(3C)<br /> | Nico21DE3plysN<br /> | 0.2 mM IPTG 25 oC o/n<br /> | Ni beads<br /> | 33KD/6.0<br /> |-<br /> | <br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu