https://eln.excellgen.com/index.php?action=history&feed=atom&title=631-pT7A3-IL-22 631-pT7A3-IL-22 - Revision history 2026-10-18T16:39:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=631-pT7A3-IL-22&diff=9404&oldid=prev Paul.xu: Created page with "== '''Cloning of 631-pT7A3-IL-22''' == '''Purpose:''' Cloning of 631-pT7A3-IL-22 '''Date: '''2013-6-5---2013-6-7 '''Notebook:006026''' '''Page:006181''' '''Clone ID:631'..." 2013-06-10T14:45:10Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 631-pT7A3-IL-22&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 631-pT7A3-IL-22 &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-5---2013-6-7 &#039;&#039;&#039;Notebook:006026&#039;&#039;&#039; &#039;&#039;&#039;Page:006181&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:631&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 631-pT7A3-IL-22&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 631-pT7A3-IL-22<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-5---2013-6-7 <br /> <br /> &#039;&#039;&#039;Notebook:006026&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:006181&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:631&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:631+651+652PCR_6-6-2013.JPG]]<br /> <br /> Gene synthesis <br /> <br /> clone into 1279 pT7A3( Nde1 + Xho1)&lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-6-7 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:631digest_6-7-2013.JPG]]&lt;br&gt; <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu