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643-pT7A3-Nhe1 - Revision history
2026-10-18T03:05:18Z
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Paul.xu at 22:24, 19 October 2018
2018-10-19T22:24:17Z
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<p><b>New page</b></p><div>== '''Cloning of 643-pT7A3-Nhe1''' ==<br />
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'''Purpose:''' Cloning of 643-pT7A3-Nhe1<br />
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'''Date: '''2013-5-24--2013-5-30<br />
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'''Notebook:0'''<br />
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'''Page:'''<br />
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'''Clone ID:643'''<br />
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'''Experimenters:''' yingying<br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[File:643+644+645clone 5-24-2013.jpg|RTENOTITLE]]<br />
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subclone from 559-pet28b-Nhe1 to 1279 pT7A3 with Nde1 + Xho1<br />
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=== Colony Screening ===<br />
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Date: 2013-5-30<br />
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Make miniprep and digest with Nde1 + Xho1<br />
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[[File:643+644 digest 5-30-2013.JPG|RTENOTITLE]]<br />
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== Protein Purification ==<br />
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[[File:643 Nhe1 QHP.jpg]]<br />
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[[File:643 Nhe1 ELISA.jpg]]<br />
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[[File:643 Nhe1 purification.JPG|RTENOTITLE]]<br />
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[[File:643 Nhe1 protein miniprpdigestion.JPG|RTENOTITLE]]<br />
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No Nhe1 activity<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
Paul.xu