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651-pT7A30-IFN-gamma - Revision history
2026-10-18T21:36:45Z
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Paul.xu at 14:06, 18 July 2013
2013-07-18T14:06:50Z
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<p><b>New page</b></p><div>== '''Cloning of 651-pT7A30-IFN-gamma''' ==<br />
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'''Purpose:''' Cloning of 651-pT7A30-IFN-gamma <br />
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'''Date: '''2013-7-12---2013-7-17 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:651''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:651PCR_7-12-2013.jpg]]<br><br />
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gene synthesis<br><br />
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IFN-gamma-1 to IFN-gamma-12<br><br />
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[[Image:651+1768clone_7-15-2013.jpg]]<br><br />
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{| width="334" cellspacing="1" cellpadding="1" border="1" height="81"<br />
|-<br />
| lane<br><br />
| 1<br><br />
| 2<br><br />
|-<br />
| <br><br />
| PCR product<br><br />
| 1747,pT7A30-iMMLV<br><br />
|-<br />
| enzymes<br><br />
| colspan="2" | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Nde1+ Xho1<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2013-7-17 <br />
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Make miniprep and digest with; Nde1 + Xho1 <br />
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[[Image:651+1768_digest_7-17-2013.jpg]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu