https://eln.excellgen.com/index.php?action=history&feed=atom&title=673-pEC-mNhe1 673-pEC-mNhe1 - Revision history 2026-10-18T19:43:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=673-pEC-mNhe1&diff=10289&oldid=prev Paul.xu at 15:21, 23 September 2013 2013-09-23T15:21:58Z <p></p> <p><b>New page</b></p><div>= &#039;&#039;&#039;Cloning of &#039;&#039;&#039;673-pEC-mNhe1 =<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 673-pEC-mNhe1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-16---2013-9-20 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:673&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:673pcr+1845pcr+1846PCR_9-16-2013.JPG]]&lt;br&gt;<br /> <br /> gene synthesis&lt;br&gt;<br /> <br /> pcr1:mNhe1-1-1 to&amp;nbsp; mNhe1-1-14&lt;br&gt;<br /> <br /> pcr2:mNhe1-2-1&amp;nbsp; to mNhe1-2-16&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:556+673+1845PCR_9-17-2013.JPG]]&lt;br&gt;<br /> <br /> PCR: pcr1+pcr2&lt;br&gt;<br /> <br /> mNhe1-1-1 +mNhe1-2-16&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:556+673+1835clone_9-18-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;307&quot; height=&quot;69&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1454,pEC&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | HindIII + BamH1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-20<br /> <br /> Make miniprep and digest with&amp;nbsp; HindIII + BamH1&lt;br&gt; <br /> <br /> [[Image:673digest_9-20-2013.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu