Paul.xu: Created page with "Aug/2014 == Cre:
== CloneID:
Name:
Grow condition:
Induce condition: 0.15 mM IPTG, room temperature o/n
Lysed with Buffer A without Imidazole. Load ..."

2014-08-18T19:11:04Z

Created page with "Aug/2014 == Cre: == CloneID: Name: Grow condition: Induce condition: 0.15 mM IPTG, room temperature o/n Lysed with Buffer A without Imidazole. Load ..."

New page

Aug/2014

== Cre: ==

CloneID: 

Name: 

Grow condition: 

Induce condition: 0.15 mM IPTG, room temperature o/n 

Lysed with Buffer A without Imidazole. Load to 5 ml GE/Pharmacia Ni FF. 

Elute from Ni column using linear imidazole 10 to 30 mM. Combine peak fractions, dilute with buffer to lower salt conc ~50 mM NaCl, load to 20 ml SP HP column (1.6 cm ID).  proteins do not bind to SP HP, load back to 10 ml Ni HP column, eluted as a single peak, analyse protein by SDS_PAGE. Looks like protein expression is rather low 

== FLP ==

Clone ID: 2044 

[[Image:Cre Flp purification 16 8 14.jpg]]

[[Category:Protein_Purification]]

68-2044 - Revision history

Paul.xu: Created page with "Aug/2014 == Cre: == CloneID: Name: Grow condition: Induce condition: 0.15 mM IPTG, room temperature o/n Lysed with Buffer A without Imidazole. Load ..."

Paul.xu: Created page with "Aug/2014 == Cre:
== CloneID:
Name:
Grow condition:
Induce condition: 0.15 mM IPTG, room temperature o/n
Lysed with Buffer A without Imidazole. Load ..."