https://eln.excellgen.com/index.php?action=history&feed=atom&title=6X_Loading_Dye6X Loading Dye - Revision history2026-10-18T06:27:56ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=6X_Loading_Dye&diff=21564&oldid=prevPaul.xu at 14:25, 4 October 20222022-10-04T14:25:03Z<p></p>
<p><b>New page</b></p><div>== 1% Cresol Red Dye Stock yield 50 ml ==<br />
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Add 500 mg to 50 ml of ddH2O in a 50 ml tube or bottle<br />
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Shake to dissolve,Store at room temperature<br />
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== Cresol Red Loading Dye containing 0.02% Cresol Red ==<br />
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yield 100 ml of working solution<br/>Add 30 g of glycerol to ~70 ml of ddH2O<br/>Shake to dissolve<br/>Add 2 ml of 1% Cresol Red Dye or amaranth, adjust to 100 ml<br/>Shake tube to mix. Store at 4°C<br />
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== Protocol 2 ==<br />
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*300 ml glycerol (30%)<br />
*2.5 g bromophenol blue or&nbsp;'''Cresol Red '''(0.25%)<br />
*dH<sub>2</sub>O to 1000mL<br />
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== 10X Xylene Cyanol/Bromophenol Blue DNA loading buffer recipe ==<br />
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*0.025 g of Xylene Cyanol FF<br />
*0.025 g of Bromophenol Blue0.025 g cresol red (optional)<br />
*1.25 ml of 10% SDS (optional)<br />
*4 ml 0.5 M EDTA (optional)<br />
*2 ml 1 M Tris-Cl pH 7.6-8.0 (optional)<br />
*12.5 ml of glycerol<br />
*water to 20 ml Store sucrose buffers at 4 or -20C.<br />
[[Category:Buffer Recipe]]</div>Paul.xu