https://eln.excellgen.com/index.php?action=history&feed=atom&title=701-pEC-mEcoR1 701-pEC-mEcoR1 - Revision history 2026-10-18T19:43:41Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=701-pEC-mEcoR1&diff=10449&oldid=prev Paul.xu at 16:00, 21 October 2013 2013-10-21T16:00:24Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 701-pEC-mEcoR1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 701-pEC-mEcoR1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-16---2013-10-21 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:701&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:701pcr_10-16-2013.JPG]]&lt;br&gt;<br /> <br /> pcr1:mEcoR1_1_1+mEcoR1_1_6&lt;br&gt;<br /> <br /> pcr2:mEcoR1_1_7+mEcoR1_1_16&lt;br&gt;<br /> <br /> pcr3:mEcoR1_2_1+mEcoR1_2_6&lt;br&gt;<br /> <br /> pcr4:mEcoR1_2_7+mEcoR1_2_16&lt;br&gt;<br /> <br /> [[Image:701+1869PCR_10-17-2013.JPG]]&lt;br&gt;<br /> <br /> PCR:mEcoR1_1_1+mEcoR1_2_16&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:701clone_10-17-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;269&quot; height=&quot;82&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR&lt;br&gt;<br /> | 1454,pEC&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | HindIII + BamH1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-21 <br /> <br /> Make miniprep and digest with&amp;nbsp; HindIII + BamH1 <br /> <br /> [[Image:701digest_10-21-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu