https://eln.excellgen.com/index.php?action=history&feed=atom&title=715-pT7A3E-wt-Xho1 2026-10-18T16:41:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=715-pT7A3E-wt-Xho1&diff=11102&oldid=prev 2014-01-28T15:57:59Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 715-pT7A3E-wt-Xho1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 715-pT7A3E-wt-Xho1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-6---2013-12017 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:715&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715 718 1905 1923PCR 12-4-2013.JPG]] <br /> <br /> PCR from genomic DNA&lt;br&gt;Xho1-Nde1-F &lt;br&gt;Xho1-Xho1-R&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:715+718clone 12-5-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR &lt;br&gt; <br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-17 <br /> <br /> Make miniprep and digest with Nde1 +Xho1 <br /> <br /> [[Image:715digest 12-17-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Fix of Cloning of 715-pT7A3E-wt-Xho1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 715-pT7A3E-wt-Xho1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014--1-9---2014-1-14 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:715&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715pcr 1-2-2014.JPG]]&lt;br&gt; <br /> <br /> pcr1:F primer+ Xho-fix-R&lt;br&gt; <br /> <br /> pcr2:xho-fix-F+ R primer&lt;br&gt; <br /> <br /> [[Image:715 718 1592clone 1-8-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR &lt;br&gt; <br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> ligate and transform in 1878pLysN-Plac-mXho1 competent cell ,grow in k/chl plate&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-13 <br /> <br /> Make miniprep and digest with Nde1 +Xho1 <br /> <br /> [[Image:715 724digest 1-13-2014.jpg]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Fix of Cloning of 715-pT7A3E-wt-Xho1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 715-pT7A3E-wt-Xho1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014--1-9---2014-1-14 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:715&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715pcr 1-2-2014.JPG]]&lt;br&gt; <br /> <br /> pcr1:F primer+ Xho-fix-R&lt;br&gt; <br /> <br /> pcr2:xho-fix-F+ R primer&lt;br&gt; <br /> <br /> [[Image:715 718 1592clone 1-8-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR &lt;br&gt; <br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-23 <br /> <br /> Make miniprep and digest with Nde1 +Xho1 <br /> <br /> [[Image:715digest_1-23-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu