https://eln.excellgen.com/index.php?action=history&feed=atom&title=718-pT7A3E-wt-BamH 2026-10-18T23:05:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=718-pT7A3E-wt-BamH&diff=10843&oldid=prev 2013-12-20T16:49:40Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 718-pT7A3E-wt-BamH&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 718-pT7A3E-wt-BamH <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-6---2013-12-19 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:718&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715_718_1905_1923PCR_12-4-2013.JPG]]&lt;br&gt;<br /> <br /> Wt-BamH1-Nde1-F ctagcacatatggaagtagaaaaagagttta&lt;br&gt;Wt-BamH1-Xho1-R ttgctagctcgagtttgttttcaactttatctttc&lt;br&gt;PCR from 712 ptrc-BamHIR&lt;br&gt;<br /> <br /> [[Image:715+718clone 12-5-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;274&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:718digest 12-19-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu