https://eln.excellgen.com/index.php?action=history&feed=atom&title=724-pBad24-sfGFP-T30C 2026-10-18T12:08:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=724-pBad24-sfGFP-T30C&diff=10971&oldid=prev 2014-01-15T14:28:02Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 724-pBad24-sfGFP-T30C&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 724-pBad24-sfGFP-T30C <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-8---2014-1-13 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:724&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:724_725_1940pcr_1-9-2014.JPG]]&lt;br&gt;<br /> <br /> PCR using pBad24-sfGFP as template&lt;br&gt;sfGFP-T30C-EcoR1-F &lt;br&gt;Ecoli-sfGFP20&lt;br&gt;<br /> <br /> [[Image:724_725_1940_1946_1961clone_1-9-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;278&quot; height=&quot;100&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | pBad24 545&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | EcoR1 + HindIII&lt;br&gt;<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-13 <br /> <br /> Make miniprep and digest with EcoR1 + HindIII<br /> <br /> [[Image:715_724digest_1-13-2014.jpg]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu