https://eln.excellgen.com/index.php?action=history&feed=atom&title=725-pBad24-sfGFP-T30C-A67G 725-pBad24-sfGFP-T30C-A67G - Revision history 2026-10-18T08:22:34Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=725-pBad24-sfGFP-T30C-A67G&diff=11028&oldid=prev Paul.xu: Created page with "== '''Cloning of 725-pBad24-sfGFP-T30C-A67G''' == '''Purpose:''' Cloning of 725-pBad24-sfGFP-T30C-A67G '''Date: '''2014---1-16--- '''Notebook:0''' '''Page:''' '''Clone ID..." 2014-01-17T21:38:39Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 725-pBad24-sfGFP-T30C-A67G&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 725-pBad24-sfGFP-T30C-A67G &#039;&#039;&#039;Date: &#039;&#039;&#039;2014---1-16--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 725-pBad24-sfGFP-T30C-A67G&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 725-pBad24-sfGFP-T30C-A67G<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014---1-16---<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:725&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:725pcr1_1-16-2014.jpg]]<br /> <br /> Cloning Method<br /> <br /> PCR using pBad24-sfGFP as template ~ 15 to 20 cycles&lt;br&gt;new oligo&lt;br&gt;SfGFP-T30C-A67G-F A12 &lt;br&gt;Ecoli-sfGFP20&lt;br&gt;<br /> <br /> [[Image:725_1981pcr_1-17-2014.jpg]]&lt;br&gt;<br /> <br /> gel purify PCR product<br /> <br /> then PCR again using gel purified DNA&lt;br&gt;new oligp&lt;br&gt;sfGFP-T30C-EcoR1-F &lt;br&gt;Ecoli-sfGFP20&lt;br&gt;<br /> <br /> [[Image:725clone_1-17-2014.jpg]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | pBad24 545&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 201<br /> <br /> Make miniprep and digest with <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu