https://eln.excellgen.com/index.php?action=history&feed=atom&title=726-pBAD-sfGFP-C66A 726-pBAD-sfGFP-C66A - Revision history 2026-10-18T16:44:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=726-pBAD-sfGFP-C66A&diff=11153&oldid=prev Paul.xu: Created page with "== '''Cloning of 726-pBAD-sfGFP-C66A''' == '''Purpose:''' Cloning of 726-pBAD-sfGFP-C66A '''Date: '''2014-1--30---2014-2-3 '''Notebook:0''' '''Page:''' '''Clone ID:726'''..." 2014-02-04T15:38:49Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 726-pBAD-sfGFP-C66A&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 726-pBAD-sfGFP-C66A &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1--30---2014-2-3 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:726&#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 726-pBAD-sfGFP-C66A&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 726-pBAD-sfGFP-C66A<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1--30---2014-2-3<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:726&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:726_727_2046_2060_718_2045pcr_1-31-2014.JPG]]<br /> <br /> PCR using 546 pBad24-sfGFP as template&lt;br&gt;new oligo<br /> <br /> sfGFP-C66A-F &lt;br&gt;<br /> <br /> old oligo&lt;br&gt;Ecoli-sfGFP20&lt;br&gt;<br /> <br /> [[Image:726_727_2046PCR_1-31-2014.JPG]]&lt;br&gt;<br /> <br /> Gel Purify PCR product, &lt;br&gt;then use the purified PCR fragment as template, run PCR<br /> <br /> Ecoli-sfGFP1&lt;br&gt;Ecoli-sfGFP20&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:726_727_2046clone_1-31-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | pBad24 545&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-3<br /> <br /> Make miniprep and digest with EcoR1 + HindIII <br /> <br /> [[Image:726_727digest_2-3-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu