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732-pBad24-SuperNova - Revision history
2026-10-18T08:06:17Z
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Paul.xu at 18:32, 9 April 2014
2014-04-09T18:32:05Z
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<p><b>New page</b></p><div>== '''Cloning of 732-pBad24-SuperNova''' ==<br />
<br />
'''Purpose:''' Cloning of 732-pBad24-SuperNova <br />
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'''Date: '''2014-4-4---2014-4-9 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:732''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:732pcr 4-4-2014.JPG]]<br> <br />
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pcr1:Supernova-1-1to&nbsp; Supernova-1-10<br> <br />
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pcr2:Supernova-2-1 Supernova-2-10<br> <br />
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[[Image:732 2145 2147 2152 2155 2174 2180PCR 4-7-2014.JPG]]<br> <br />
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PCR:pcr1+pcr2<br> <br />
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[[Image:732 1841 2145 2147clone 4-7-2014.JPG]]<br> <br />
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{| width="200" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| Lane<br> <br />
| 1<br> <br />
| 2<br><br />
|-<br />
| <br> <br />
| PCR<br> <br />
| 545 pBad24<br><br />
|-<br />
| <br> <br />
| colspan="2" | EcoR1 + HindIII<br><br />
|}<br />
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<br> <br />
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=== Colony Screening ===<br />
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Date: 2014-4-9 <br />
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Make miniprep and digest with EcoR1 + HindIII<br> <br />
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[[Image:732_1841digest_4-9-2014.JPG]]<br> <br />
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<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu