https://eln.excellgen.com/index.php?action=history&feed=atom&title=734_pLike-rep-Not1 734 pLike-rep-Not1 - Revision history 2026-10-18T12:33:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=734_pLike-rep-Not1&diff=12571&oldid=prev Excellgen at 20:13, 28 July 2014 2014-07-28T20:13:14Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 734 pLike-rep-Not1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 734pLike-rep-Not1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:734&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 567&lt;br&gt;Not1-Xba1-F&lt;br&gt;atacactctagaTGCGTAGCGATACCAGCGTTG&lt;br&gt; <br /> <br /> Not1-HindIII-R&lt;br&gt;(CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT)&lt;br&gt;AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG&lt;br&gt; <br /> <br /> [[Image:2264 734 2279 2273 2268 2278-2pcr.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> digest with Xba1 +HindIII, clone to 2270 pLIKE-rep&lt;br&gt; <br /> <br /> [[Image:Digest for 734 2279 2264 2015.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_2_14 <br /> <br /> Make miniprep, use&amp;nbsp; Xba1 +HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:734_2279digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen