https://eln.excellgen.com/index.php?action=history&feed=atom&title=736_pEC2-mNco1 736 pEC2-mNco1 - Revision history 2026-10-18T08:07:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=736_pEC2-mNco1&diff=13917&oldid=prev Excellgen at 21:36, 30 January 2015 2015-01-30T21:36:43Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 736 pEC2-mNco1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of736 pEC2-mNco1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:736&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> gene synthesis <br /> <br /> [[Image:736 2351pcr.JPG]] <br /> <br /> jion two fragments by PCR <br /> <br /> [[Image:736 2351pcr-C.JPG]] <br /> <br /> DIGEST WITH NdeI+XhoI <br /> <br /> [[Image:DIGEST FOR736 2351.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use NdeI + XhoI to find positive clone&lt;br&gt; <br /> <br /> [[Image:736 2351DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === fix 736-5 pEC2-mNco1 ===<br /> <br /> pcr &lt;br&gt; <br /> <br /> [[Image:736fix 1916fix2 2323pcr.JPG]][[Image:736fix 1916fix2 2323pcr2.JPG]] <br /> <br /> jOIN two fragment by PCR <br /> <br /> [[Image:736fix 1916fix2 1917 2240.JPG]]&lt;br&gt; digest with Nde1 + XhoI&lt;br&gt; <br /> <br /> [[Image:Digest for2354 2369 736fix2 1916fix3 2240.JPG]][[Image:Digest for736fix2 1916fix3 2240.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:736FIX2 1916FIX3 2240 2354 2369 2375.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> === fix 736-5 pEC2-mNco1 ===<br /> <br /> pcr&amp;nbsp; with Tth-muts&lt;br&gt; <br /> <br /> [[Image:2401-1 2198 2229-1 736fix3-1pcr.JPG]][[Image:2229-2 736fix3-2pcr.JPG]]&lt;br&gt; <br /> <br /> jOIN two fragment by PCR <br /> <br /> [[Image:2229 736fix3pcr.JPG]]&lt;br&gt; digest with Nde1 + XhoI&lt;br&gt; <br /> <br /> [[Image:Digest for 736fix3 2198 2229 2416.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nde1 + PST1 to find positive clone&lt;br&gt; [[Image:736fix3 2198 2229digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen