https://eln.excellgen.com/index.php?action=history&feed=atom&title=757_2398_2408_2392 757 2398 2408 2392 - Revision history 2026-10-18T15:06:27Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=757_2398_2408_2392&diff=13900&oldid=prev Excellgen: Created page with "== Protein Expression == 2015/1/23<br> SHANYI Image:757_2398_2408_2392.jpg<br> {| cellspacing="1" cellpadding="1" border="1" width="832" |- | Lane<br> | Name<br> | Pr..." 2015-01-27T17:57:43Z <p>Created page with &quot;== Protein Expression == 2015/1/23&lt;br&gt; SHANYI <a href="/index.php?title=File:757_2398_2408_2392.jpg" title="File:757 2398 2408 2392.jpg">Image:757_2398_2408_2392.jpg</a>&lt;br&gt; {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;832&quot; |- | Lane&lt;br&gt; | Name&lt;br&gt; | Pr...&quot;</p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2015/1/23&lt;br&gt; SHANYI <br /> <br /> [[Image:757_2398_2408_2392.jpg]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;832&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | SAMPLE&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | express&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;4&quot; | pEB3-Nco1 &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;4&quot; | Nco1 &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;4&quot; | &lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;2&quot; | &lt;br&gt; &lt;br&gt;15 ml,<br /> Tuner(DE3)plysN-hia5<br /> <br /> | rowspan=&quot;4&quot; | &lt;br&gt; 0.2%Arabinose<br /> RT O/N &lt;br&gt;<br /> <br /> | s&lt;br&gt; <br /> | <br /> 5.8/32.4&lt;br&gt; <br /> <br /> | no&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | p&lt;br&gt; <br /> | &lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 15 ml,<br /> Top10plysN-hia5&lt;br&gt;<br /> <br /> | S&lt;br&gt; <br /> | &lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | p&lt;br&gt; <br /> | &lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | pageRuler Plus Prestained protein ladder&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; &lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | &amp;nbsp;pMCSG7-hRAD52&lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> &lt;br&gt; 2398&lt;br&gt; <br /> <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | 0.4mM IPTG RT O/N <br /> | s&lt;br&gt; <br /> | &lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | rowspan=&quot;4&quot; | pL42-T4-uvsY&lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> | &lt;br&gt; <br /> | 2408-3&lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;4&quot; | 42oc 4hours &lt;br&gt; &lt;br&gt;<br /> | s&lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | no&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; &lt;br&gt; <br /> | 15 ml, <br /> Tuner(DE3)plysN&lt;br&gt; <br /> <br /> | s&lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | no&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | &lt;br&gt; <br /> | 2408-7&lt;br&gt; <br /> | <br /> &amp;nbsp;15 ml, <br /> <br /> Tuner(DE3)plysN &lt;br&gt; <br /> <br /> | s&lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | no&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | S&lt;br&gt; <br /> | &lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | pEB3-uvsX&lt;br&gt; <br /> | &lt;br&gt; <br /> | 2392&lt;br&gt; <br /> | 1L<br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | 0.1%Arabinose<br /> 27oC O/N &lt;br&gt;<br /> <br /> | S&lt;br&gt; <br /> | &lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN <br /> <br /> &lt;br&gt; <br /> <br /> | 0.2%Arabinose<br /> 27oC O/N &lt;br&gt;<br /> <br /> | s&lt;br&gt; <br /> | &lt;br&gt; <br /> | yes&lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Excellgen