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758 pEc2-PacI - Revision history
2026-10-18T16:02:48Z
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Excellgen at 21:35, 13 January 2015
2015-01-13T21:35:45Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of758 pEc2-PacI''' ==<br />
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'''Purpose:''' Cloning of758 pEc2-PacI'''==''' <br />
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'''Date: '''2014-12_2015_1 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:758''' <br />
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'''Experimenters:'''&nbsp; shanyi <br />
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== '''Results''' ==<br />
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=== CLONING ===<br />
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gene synthesis <br />
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[[Image:758pcr.JPG]] <br />
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clone to 2015 using Nde1 + Xho1 <br />
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[[Image:Digest for 758.JPG]]<br> <br />
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=== Colony Screening ===<br />
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Date: 2015_1<br> <br />
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Make miniprep, use Nde1 + Xho1 to find positive clone<br> <br />
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<br> [[Image:2391fix 758 760 761 757 )2392digest.JPG]]<br> <br />
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<br> <br />
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=== Fix 758-2 and 758-3 ===<br />
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pcr <br><br />
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fragment 1 using 758-2 as template<br><br />
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758-PacI-1+758-PacI-4<br><br />
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fragment 1 using 758-3 as template<br />
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758-PacI-5+758-PacI-12<br><br />
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[[Image:758fix_760fix_2403_2401-2pcr.JPG]]<br> <br />
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clone to 2015 using Nde1 + Xho1 <br />
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[[Image:758fix_760fix_2401_2321_2399pcr.JPG]]<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2015_1<br> <br />
<br />
Make miniprep, use Nde1 + Xho1 to find positive clone<br> <br />
<br />
[[Image:758fix_760fix_2394_2400_2401_2402_2404_2403.JPG]]<br> <br> <br />
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== '''Result intepretation/Summary''' ==<br />
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*<br><br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
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