https://eln.excellgen.com/index.php?action=history&feed=atom&title=761_pEC2-SwaI 761 pEC2-SwaI - Revision history 2026-10-18T06:31:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=761_pEC2-SwaI&diff=13800&oldid=prev Excellgen: Created page with "== '''Cloning of761 pEC2-SwaI''' == '''Purpose:''' Cloning of761 pEC2-SwaI'''==''' '''Date: '''2014-12_2015_1 '''Notebook:0''' '''Page:''' '''Clone ID:761''' '''Exper..." 2015-01-08T17:51:45Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of761 pEC2-SwaI&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of761 pEC2-SwaI&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-12_2015_1 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:761&#039;&#039;&#039; &#039;&#039;&#039;Exper...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of761 pEC2-SwaI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of761 pEC2-SwaI&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-12_2015_1 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:761&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> gene synthesis <br /> <br /> [[Image:760 761 2391fixpcr.JPG]]&lt;br&gt; <br /> <br /> clone to 2015 using Nde1 + Xho1 <br /> <br /> [[Image:Digest for 760 761.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> Make miniprep, use Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; [[Image:2391fix 758 760 761 757 )2392digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen