https://eln.excellgen.com/index.php?action=history&feed=atom&title=7_mRNA_Stefania 7 mRNA Stefania - Revision history 2026-10-18T10:26:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=7_mRNA_Stefania&diff=4716&oldid=prev Paul.xu at 13:28, 18 June 2012 2012-06-18T13:28:12Z <p></p> <p><b>New page</b></p><div>mRNA Production for Stefania <br /> <br /> 6/8/2012 <br /> <br /> PCR amplification using plasmids from Stefania <br /> <br /> [[Image:1211 PCR 6-5-2012.JPG]] <br /> <br /> [[Image:1212 1215 1216 PCR 6-8-2012.JPG]] <br /> <br /> [[Image:1209 1212 1214 1215 1216 PCR 6-8-2012.JPG]] <br /> <br /> [[Image:1210 1213 PCR 6-8-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> 2012/6/11 <br /> <br /> 1209---1216 digest result:&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 689px; height: 80px;&quot;<br /> |-<br /> | <br /> Lane&lt;br&gt; <br /> <br /> | 1&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 11&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 15&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | xba1+Not1&lt;br&gt; <br /> | xba1&lt;br&gt; <br /> | xba1+sal1&lt;br&gt; <br /> | xba1+sal1&lt;br&gt; <br /> | xba1&lt;br&gt; <br /> | EcoR1+Pst1&lt;br&gt; <br /> | xba1+sal1&lt;br&gt; <br /> | xba1&lt;br&gt; <br /> | xba1+sal1&lt;br&gt;<br /> |-<br /> | ID&lt;br&gt; <br /> | colspan=&quot;3&quot; | 1209pMXs-Lhx3&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1214pMXs-Isl1&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1210Tet-O-FUW-FoxG&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1216pMXs-Brn2&lt;br&gt;<br /> |}<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 622px; height: 80px;&quot;<br /> |-<br /> | Lane <br /> | 6&lt;br&gt; <br /> | 12&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 13&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 14&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | xba1&lt;br&gt; <br /> | xba1+sal1&lt;br&gt; <br /> | xba1&lt;br&gt; <br /> | EcoR1+xba1&lt;br&gt; <br /> | xba1&lt;br&gt; <br /> | xba1+sal1&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | ID&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1211pMXs-MYT1L&lt;br&gt; <br /> | 1212pMXs-Ngn2&lt;br&gt; <br /> | 1213pCS2-MT GLI2 FL&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1215pMXs-Hb9&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:1209--1216 digest 6-11-2012.JPG]]&lt;br&gt;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; 2012/6/12 PCR: <br /> <br /> [[Image:1211 1212 1213 1215 1216 PCR 6-12-2012.JPG]] <br /> <br /> 1211,1212,1215,1216 sizes are right <br /> <br /> [[Image:1213PCR 6 13 2012.JPG]] <br /> <br /> 1213 size right&lt;br&gt; <br /> <br /> [[Image:1209 1210 1211 1212PCR 6-13-2012.JPG]] <br /> <br /> 1209,1211 size right <br /> <br /> [[Image:1213 1214 1215 1216PCR 6-14-2012.JPG]] <br /> <br /> 1214 size right <br /> <br /> [[Image:1210 1212 1215 1216PCR 6-14-2012.JPG]] <br /> <br /> 1216 size right <br /> <br /> [[Image:1210_1212_1215_1216PCR_6_18-2012.JPG]]<br /> <br /> size are right<br /> <br /> &lt;br&gt; <br /> <br /> Clones replacement: <br /> <br /> pMXs-Brn2 ==&amp;gt;pCMV6-POU3F2&lt;br&gt;pGEM-ISLET1==&amp;gt;pMXs-Isl1&lt;br&gt; <br /> <br /> BHLHB9: no growth&lt;br&gt; &lt;br&gt; <br /> <br /> [[Category:Services]]</div> Paul.xu