https://eln.excellgen.com/index.php?action=history&feed=atom&title=876_pJet1.2-Dr-Muts 2026-10-18T11:50:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=876_pJet1.2-Dr-Muts&diff=16441&oldid=prev 2017-09-01T20:20:27Z

<p></p> <p><b>New page</b></p><div>PCR 3 fragments from genomic DNA&lt;br/&gt;Fragment 1<br /> <br /> 874-Nde1-F2&lt;br/&gt;CAGCGGCCTGGTGCCGCGCGGCAGC CATATGCGAGCAGTTTCCCCCACCAAAG<br /> <br /> 874-R1&lt;br/&gt;TCGCCGAGCGTTTCCCGAATCTCAC&lt;br/&gt;(GTGAGATTCGGGAAACGCTCGGCGA)<br /> <br /> &lt;br/&gt;Fragment 2:&lt;br/&gt;874-seq-F1: TGA GAT TCG GGA AAC GCT C &amp;nbsp;<br /> <br /> 847-R2&lt;br/&gt;GGGCGCGGTCTTTGAGGGTCGCAATC&lt;br/&gt;(GATTGCGACCCTCAAAGACCGCGCCC)<br /> <br /> Fragment 3:&lt;br/&gt;874-seq-F2: AGA TTG CGA CCC TCA AAG AC&amp;nbsp;&lt;br/&gt;874-Sal1-R2&lt;br/&gt;(CTGGGGGAGGGCCATGAATCCCGG GTCGACAAGCTTGCGGCCGCACTCGAGCACC)&lt;br/&gt;GGTGCTCGAGTGCGGCCGCAAGCTTGTCGACCCGGGATTCATGGCCCTCCCCCAG<br /> <br /> <br /> <br /> <br /> <br /> 1st PCR<br /> <br /> [[File:876 1st PCR.JPG|RTENOTITLE]]<br /> <br /> 2nd PCR<br /> <br /> mix 3 fragments, and gel purified, PCR using<br /> <br /> 874-Nde1-F2&lt;br/&gt;CAGCGGCCTGGTGCCGCGCGGCAGC CATATGCGAGCAGTTTCCCCCACCAAAG<br /> <br /> 874-Sal1-R2&lt;br/&gt;(CTGGGGGAGGGCCATGAATCCCGG GTCGACAAGCTTGCGGCCGCACTCGAGCACC)&lt;br/&gt;GGTGCTCGAGTGCGGCCGCAAGCTTGTCGACCCGGGATTCATGGCCCTCCCCCAG<br /> <br /> [[File:876 PCR.JPG|RTENOTITLE]]<br /> <br /> PCR screening using&amp;nbsp;<br /> <br /> 874-seq-F1: TGA GAT TCG GGA AAC GCT C &amp;nbsp;<br /> <br /> 847-R2&lt;br/&gt;GGGCGCGGTCTTTGAGGGTCGCAATC&lt;br/&gt;(GATTGCGACCCTCAAAGACCGCGCCC)<br /> <br /> [[File:876 PCR Screening.JPG|RTENOTITLE]]<br /> <br /> miniprep digest with Nde1+ Xho1<br /> <br /> [[File:876 digestion.JPG|RTENOTITLE]]<br /> <br /> [[File:876 digestion l.JPG|RTENOTITLE]]<br /> <br /> [[File:876 digestion 2.JPG|RTENOTITLE]]</div>

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