https://eln.excellgen.com/index.php?action=history&feed=atom&title=940_pET28b-yPolyA-Po
940 pET28b-yPolyA-Po - Revision history
2026-10-18T15:41:23Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=940_pET28b-yPolyA-Po&diff=16828&oldid=prev
Crm at 17:32, 29 January 2018
2018-01-29T17:32:20Z
<p></p>
<p><b>New page</b></p><div>938 digestion with Nde1-Xho1, Fastdigest Buffer<br />
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[[File:938 939 942 3122 931 1855 digestion.JPG|RTENOTITLE]]<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width: 681px;"<br />
|-<br />
| 1<br />
| 2<br />
| 3<br />
| 4<br />
| 5<br />
| 6<br />
| 7<br />
| style="width: 54px;" | 8,9<br />
| style="width: 55px;" | 10,11<br />
| style="width: 71px;" | 13<br />
|-<br />
| 938&nbsp;PNGase_F<br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">939 Tte-ExoIII</span><br/><br />
| 942 VVN<br/><br />
| 3122 CRM197<br/><br />
| 931, 1, PAK16D ligase&nbsp;<br/><br />
| 931, 2, PAK16D ligase<br/><br />
| 1855&nbsp; yPolyA-Pol<br/><br />
| style="width: 54px;" | 1540<br />
| style="width: 55px;" | 2046<br />
| style="width: 71px;" | 2046<br/><br />
|-<br />
| Nde1-Xho1<br />
| Nde1-Xho1<br/><br />
| <span style="color:#FF0000;">Nco1-</span>Xho1<br/><br />
| Nde1-Xho1<br/><br />
| Nde1-<span style="color:#FF0000;">HindIII</span><br/><br />
| <span style="color:#FF0000;">HindIII</span>-Xho1<br />
| Nde1-Xho1<br />
| style="width: 54px;" | Nde1-Xho1<br />
| style="width: 55px;" | Nde1-Xho1<br />
| style="width: 71px;" | <span style="color:#FF0000;">Nco1</span>-Xho1<br />
|-<br />
| pJet1.2<br />
| pJet1.2<br/><br />
| pJet1.2<br/><br />
| pJet1.2<br/><br />
| pJet1.2<br/><br />
| pJet1.2<br/><br />
| pEC2-<br/><br />
| style="width: 54px;" | pET30b<br />
| style="width: 55px;" | pET28b<br />
| style="width: 71px;" | pET28b<br/><br />
|-<br />
| 1145<br/><br />
| 780<br/><br />
| 716<br/><br />
| 1798<br/><br />
| 1174<br/><br />
| 986<br/><br />
| 1713<br />
| style="width: 54px;" | there is no Xho1 site<br />
| style="width: 55px;" | 1377<br />
| style="width: 71px;" | ~1377<br />
|-<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| style="width: 54px;" | <br/><br />
| style="width: 55px;" | <br/><br />
| style="width: 71px;" | <br/><br />
|}<br />
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[[File:938 939 942 3122 931 1855 digestion Cutsmart.JPG|RTENOTITLE]]<br />
<br />
940 in pET28b<br />
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1/29/18<br />
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[[File:940 3001 3009 3135 digestion2.JPG]]<br />
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[[Category:Cloning]]</div>
Crm