https://eln.excellgen.com/index.php?action=history&feed=atom&title=941_pT7A3-pMBP-T7-Endo-1 941 pT7A3-pMBP-T7-Endo-1 - Revision history 2026-10-18T05:51:21Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=941_pT7A3-pMBP-T7-Endo-1&diff=18875&oldid=prev Crm at 20:36, 27 July 2018 2018-07-27T20:36:26Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 941 pT7A3-pMBP-T7-Endo-1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 941 pT7A3-pMBP-T7-Endo-1<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;06, 28, 2018<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 941&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone from 1225<br /> <br /> &#039;&#039;&#039;941subclone&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;vector 947&#039;&#039;&#039;<br /> <br /> [[File:IMG 1014.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;redo&#039;&#039;&#039;<br /> <br /> [[File:IMG 10121.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;Miniprep of 941&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Vector<br /> | Clone # Name<br /> | Method<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 941<br /> | 1-5<br /> | 947<br /> | 12-15;16 is+<br /> | subclone<br /> | Nde1+Xho1<br /> | 456bp<br /> | #2,#4 might be good<br /> |}<br /> <br /> <br /> <br /> &#039;&#039;&#039;Miniprep digestion _3205_3215_941&#039;&#039;&#039;<br /> <br /> [[File:IMG 1032.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> redo<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 941_3244_998&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Vector<br /> | Method<br /> | style=&quot;width: 106px;&quot; | Enzymes<br /> | style=&quot;width: 34px;&quot; | Size<br /> | style=&quot;width: 34px;&quot; | Note<br /> |-<br /> | 941<br /> | 1-4; 5&amp;nbsp; is + control<br /> | 947<br /> | Subclone<br /> | style=&quot;width: 106px;&quot; | Nde1+Xho1<br /> | style=&quot;width: 34px;&quot; | 456bp<br /> | style=&quot;width: 34px;&quot; | &lt;br/&gt;<br /> |-<br /> | 3244<br /> | 6-9<br /> | 3242<br /> | PCR cloning<br /> | style=&quot;width: 106px;&quot; | Nde1+HindIII<br /> | style=&quot;width: 34px;&quot; | 739bp<br /> | style=&quot;width: 34px;&quot; | &lt;br/&gt;<br /> |-<br /> | 998<br /> | 10-13<br /> | 1708<br /> | Gibson Assembly<br /> | style=&quot;width: 106px;&quot; | HindIII+Xho1<br /> | style=&quot;width: 34px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 34px;&quot; | &lt;br/&gt;<br /> |}<br /> <br /> [[File:IMG 1213.JPG|RTENOTITLE]]<br /> [[Category:Cloning]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==</div> Crm