https://eln.excellgen.com/index.php?action=history&feed=atom&title=948_pJet1.2-ccdb 2026-10-18T11:02:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=948_pJet1.2-ccdb&diff=16872&oldid=prev 2018-02-03T21:38:30Z

<p></p> <p><b>New page</b></p><div>948, not 947 digest with EcoRV<br /> <br /> [[File:948 EcoRV 2.JPG|RTENOTITLE]]<br /> <br /> [[File:948 EcoRV.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> [[File:948 Xho1 Xba1 950 Xho1.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> = == =<br /> <br /> Date 1/22/2018<br /> <br /> Digest miniprep DNA (in pJect1.2 vector) with Xho1-Xba1, (Cloning sites Xho1-Xba1)<br /> <br /> [[File:3001 3009 3135 950 948 digestion.JPG|RTENOTITLE]]<br /> <br /> [[File:948-3135-3009-3001-950 digestion2.JPG.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> 948 vector, digest with EcoRV&amp;nbsp;+&amp;nbsp; CIP<br /> <br /> ligate with PCR product, make miniprep, digest with Xba1+ Xho1<br /> <br /> [[File:948 cloning CIP EcoRV.JPG]]<br /> <br /> [[File:948 ligation.jpg]]</div>

Paul.xu