Crm at 18:35, 5 June 2018

2018-06-05T18:35:46Z

New page

== Date:4/2/2018 ==

'''subclone of 984'''

Vector:1987

Insert:982

Enzymes:Xba1-Nhe1

[[File:IMG 0604.JPG|RTENOTITLE]]

== Date:4/4/2018 ==

'''miniprep digestion of 984_947_3152'''

#Name Enzymes Size:

1-4:984 XbaI+NheI                     957bp

5 :982(973-1pMiniT) XbaI+NheI

6-9:947 XbaI+NcoI-HF                1344bp

10:947pMiniT XbaI+NcoI-HF

11:947(from PCR cloning) XbaI+NcoI-HF

12-15:3152 BsrGI+NheI              371bp

16: 3162(Vector) BsrGI+NheI

[[File:IMG 0615.JPG|RTENOTITLE]]

== Date:4/5/2018 ==

'''Subclone 984'''

Vector:1987

Insert size:957 bp

[[File:Subclone 984.JPG|RTENOTITLE]]

'''subclone of 984'''

Vector:1987

Insert:982

Size:957 bp

Enzymes:Xba1-Nhe1

[[File:IMG 0604.JPG|RTENOTITLE]]

=== Date:5/29/2018 ===

'''Subclone and Vector digestion'''

[[File:IMG 0860.JPG|RTENOTITLE]]

'''Miniprep digestion of 984_931'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| Enzymes
| Size
| Note
|-
| 984
| 2-5
| Xba1+Nhe1
| 957bp
| '''#6,7,,8 seems good'''
|-
| 931
| 1,6-8
| HindIII+Xho1
| 986bp
| '''#5 seems good'''
|}

<span style="color:#FF0000;">samples are mislabled?</span>

[[File:IMG 0871.JPG|RTENOTITLE]] <span style="color:#FF0000;">not good.</span>sequencing sample:   clone #, result: Junk

==Date:5/6/2018==
'''Miniprep of 3191_984_995'''

[[File:IMG 0905.JPG|RTENOTITLE]]

[[Category:Cloning]]

984 pT7A3-6H-SBP-SUMO-3C - Revision history

Crm at 18:35, 5 June 2018