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994 pT7A3-GST-CD133-EC2 - Revision history
2026-10-18T11:23:09Z
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Crm: Created page with "'''Vector digestion''' RTENOTITLE '''PCR of 994_995''' RTENOTITLE ''Miniprep digestion of 998_1000_994_995''' {| border="1" ce..."
2018-07-11T19:25:49Z
<p>Created page with "'''Vector digestion''' <a href="/index.php?title=File:IMG_0858.JPG" title="File:IMG 0858.JPG">RTENOTITLE</a> '''PCR of 994_995''' <a href="/index.php?title=File:IMG_0856.JPG" title="File:IMG 0856.JPG">RTENOTITLE</a> ''Miniprep digestion of 998_1000_994_995''' {| border="1" ce..."</p>
<p><b>New page</b></p><div>'''Vector digestion'''<br />
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[[File:IMG 0858.JPG|RTENOTITLE]]<br />
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'''PCR of 994_995'''<br />
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[[File:IMG 0856.JPG|RTENOTITLE]]<br />
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''Miniprep digestion of 998_1000_994_995'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Enzymes<br />
| Size<br />
| Note<br />
|-<br />
| 998<br />
| 1-4<br />
| HindIII+Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| 1000<br />
| 5-8<br />
| HIndIII+Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| 994<br />
| 9-12<br />
| BamH1+Xho1<br />
| 616<br />
| <br/><br />
|-<br />
| 995<br />
| 13-16<br />
| BamH1+Xho1<br />
| <br/><br />
| <br/><br />
|}<br />
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'''''[[File:IMG 0873.JPG|RTENOTITLE]]'''''<br />
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994: no good<br />
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== Date:6/18/2018 ==<br />
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'''Vector digestion'''<br />
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[[File:IMG 0957.JPG|RTENOTITLE]]<br />
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== '''Cloning of''' ==<br />
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'''Purpose:''' Cloning of 994 pCold-GST-CD133-EC2<br />
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'''Date: '''06, 29, 2018<br />
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'''Notebook:&nbsp;'''<br />
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'''Page:'''<br />
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'''Clone ID: 994'''<br />
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'''Experimenters: '''yijia<br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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PCR from 3147 pANT7_cGST-PROM1<br />
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'''PCR994'''<br />
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[[File:PCR994 (2).JPG|RTENOTITLE]]<br />
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'''Vector &nbsp;is 3225pT7A3-GST-MICA&nbsp;''''''digestion'''<br />
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[[File:IMG 1016.JPG|RTENOTITLE]]<br />
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=== &nbsp;Colony screen ===<br />
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'''Miniprep of 129_994_1000_3206'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Vector<br />
| Lane<br />
| Enzymes<br />
| Size<br />
| Clone#<br />
| Note<br />
|-<br />
| 129<br />
| 1801<br />
| 1-4<br />
| Nde1+HindIII<br />
| 2433<br />
| 2,4 look good<br />
| '''129-2;129-4'''<br />
|-<br />
| 994<br />
| 3225<br />
| 5-8<br />
| BamH1+Xho1<br />
| 609<br />
| 5,6,look good<br />
| Vecotor doesn't have BamH1 Site,'''994-1;994-2'''<br />
|-<br />
| 1000<br />
| 1708<br />
| 9-12<br />
| HIndIII+Xho1<br />
| 118<br />
| 11,12 looks good<br />
| '''1000-3;1000-4'''<br />
|-<br />
| 3206<br />
| 3165<br />
| 13-16<br />
| HIndIII+BamH1<br />
| 1460<br />
| <br/><br />
| need to do PCR screening<br />
|}<br />
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[[File:IMG 1053.JPG|RTENOTITLE]]<br />
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Sequencing: 994 #1&nbsp;missing 1 T, need to screen plasmids using Nde1 -Xho1<br />
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'''Miniprep digestion of 994'''<br />
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[[File:IMG 1075.JPG|RTENOTITLE]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
Crm