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995 pCold-GST-CD133-EC3 - Revision history
2026-10-18T10:29:11Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=995_pCold-GST-CD133-EC3&diff=18681&oldid=prev
Crm at 23:07, 12 July 2018
2018-07-12T23:07:11Z
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<p><b>New page</b></p><div>'''Vector digestion'''<br />
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[[File:IMG 0858.JPG|RTENOTITLE]]<br />
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'''PCR of 994_995'''<br />
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[[File:IMG 0856.JPG|RTENOTITLE]]<br />
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''Miniprep digestion of 998_1000_994_995'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Enzymes<br />
| Size<br />
| Note<br />
|-<br />
| 998<br />
| 1-4<br />
| HindIII+Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| 1000<br />
| 5-8<br />
| HIndIII+Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| 994<br />
| 9-12<br />
| BamH1+Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| 995<br />
| 13-16<br />
| BamH1+Xho1<br />
| 528<br />
| <br/><br />
|}<br />
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'''''[[File:IMG 0873.JPG|RTENOTITLE]]'''''995: no good<br />
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== Date:6/5/2018 ==<br />
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'''Miniprep of 3191_984_995'''<br />
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[[File:IMG 0905.JPG|RTENOTITLE]]<br />
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'''Miniprep digestion of 3218_995_3197'''<br />
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Vector is 3225<br />
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[[File:IMG 1048.JPG|RTENOTITLE]] sequencing:&nbsp;995 #1 is junk<br />
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'''995PCR'''<br />
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[[File:IMG 1076.JPG|RTENOTITLE]]<br />
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'''Miniprep digestion of 931-1_995'''<br />
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[[File:IMG 1102.JPG|RTENOTITLE]]<br />
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== Redo ==<br />
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Date:7/10/2018<br />
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'''995 PCR'''<br />
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Template:3147<br />
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Size:615bp<br />
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Size: [[File:IMG 1111.JPG|RTENOTITLE]]<br />
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'''995 PCR screening'''<br />
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[[File:IMG 1123.JPG]]<br />
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[[Category:Cloning]]</div>
Crm