https://eln.excellgen.com/index.php?action=history&feed=atom&title=997_pT7A3-Qbeta
997 pT7A3-Qbeta - Revision history
2026-10-18T05:40:59Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=997_pT7A3-Qbeta&diff=17736&oldid=prev
Crm at 20:30, 19 April 2018
2018-04-19T20:30:37Z
<p></p>
<p><b>New page</b></p><div>== Date:4/6/2018 ==<br />
<br />
'''redo PCR of 3127'''<br />
<br />
new primer<br />
<br />
3127-HindIII-R<br />
<br />
3127-NheI-F2<br />
<br />
size:403bp<br />
<br />
extension time:30s<br />
<br />
[[File:IMG 0612.JPG|RTENOTITLE]]<br />
<br />
'''3127 subclone'''<br />
<br />
'''Size:408 bp'''<br />
<br />
'''Insert:939pJet1.2'''<br />
<br />
'''Vector:1727 pT7A30'''<br />
<br />
'''Enzymes:Nde1+HindIII'''<br />
<br />
[[File:3127subclone.JPG|RTENOTITLE]]<br />
<br />
'''Miniprep of 944_3166_3127'''<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Size<br />
| Enzymes<br />
| Result<br />
| Note<br />
|-<br />
| 944<br />
| 1-4; 5 is 944pminiT2<br />
| 2334bp<br />
| Nde1+Xho1<br />
| Size is a little bigger than 2334 bp, need to do sequencing<br />
| 944pMiniT2 is from PCR<br />
|-<br />
| 3166<br />
| 5-9; 10 is 3166pminiT2<br />
| 1234bp<br />
| BsrG1+Sal1<br />
| to do sequence<br />
| 3166pMiniT2 is from PCR<br/><br />
|-<br />
| 3127<br />
| 11-14; 14 is 3127pMiniT2<br />
| 402bp<br />
| Nde1+HindIII<br />
| to do sequence<br />
| 3127pMiniT2 is from PCR<br />
|}<br />
<br />
[[File:IMG 0684.JPG|RTENOTITLE]]<br />
<br />
<br />
<br />
Result:Because used the vector pT7A3 not pET28b-qBeta, so name this a new clone&nbsp; as 997 and redo 3127</div>
Crm