https://eln.excellgen.com/index.php?action=history&feed=atom&title=999_pT7A30-SssI 999 pT7A30-SssI - Revision history 2026-10-18T17:25:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=999_pT7A30-SssI&diff=18877&oldid=prev Crm at 20:43, 27 July 2018 2018-07-27T20:43:58Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of&amp;nbsp;&#039;&#039;&#039;&lt;span dir=&quot;auto&quot;&gt;SssI into&amp;nbsp;pT7A30&lt;/span&gt; ==<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Purpose:&#039;&#039;&#039;&amp;nbsp;&amp;nbsp;&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Date:&amp;nbsp; 07/09/2018&#039;&#039;&#039;&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Page:&#039;&#039;&#039;&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Clone ID: 999&#039;&#039;&#039;&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; yijia&lt;/span&gt;<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === &#039;&#039;&#039;Subcloning&#039;&#039;&#039; ===<br /> <br /> Date: 7/&amp;nbsp;6 /2018<br /> <br /> By:&amp;nbsp;&amp;nbsp;<br /> <br /> parent (insert):1916pMiniT2<br /> <br /> vector:1873<br /> <br /> Gel:<br /> <br /> [[File:IMG 1081.JPG|RTENOTITLE]]<br /> <br /> [[File:IMG 1096.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> &#039;&#039;&#039;&amp;nbsp;&#039;&#039;&#039;Ligation: 30 min with T4 DNA ligase<br /> <br /> Competent cells: NEBTurbo<br /> <br /> == redo 2nd time ==<br /> <br /> &#039;&#039;&#039;999 subclone&#039;&#039;&#039;<br /> <br /> [[File:IMG 1164.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;&amp;nbsp;&#039;&#039;&#039;<br /> <br /> <br /> <br /> <br /> <br /> &#039;&#039;&#039;Restriction Digestion&#039;&#039;&#039;<br /> <br /> Date:&amp;nbsp; 7/13/2018<br /> <br /> By:&amp;nbsp; Yijia<br /> <br /> &#039;&#039;&#039;miniprep digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 1128.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> &#039;&#039;&#039;miniprep digestion 3225_999_931&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Vector<br /> | Enyzmes<br /> | Size<br /> | note<br /> |-<br /> | 3225<br /> | 1-4; 5 is + control<br /> | 1851 pT7A3-GST<br /> | Nhe1+Xho1<br /> | 383bp<br /> | &lt;br/&gt;<br /> |-<br /> | 999<br /> | 6-9;10 is +control<br /> | 1873<br /> | Nde1+Xho1<br /> | 1182bp<br /> | &lt;br/&gt;<br /> |-<br /> | 931<br /> | 11-14;15 is + control<br /> | 931-1<br /> | HindIII+Xho1<br /> | &lt;br/&gt;<br /> | failed<br /> |}<br /> <br /> [[File:IMG 1211.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Crm