https://eln.excellgen.com/index.php?action=history&feed=atom&title=Agarose-Gel-ElectrophoresisAgarose-Gel-Electrophoresis - Revision history2026-10-18T03:14:13ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Agarose-Gel-Electrophoresis&diff=986&oldid=prevPaul.xu at 16:29, 9 June 20112011-06-09T16:29:39Z<p></p>
<p><b>New page</b></p><div>1. Make 1% gel <br />
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1.6 g agarose (for electrophoresis). <br />
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160 ml 0.5 X TAE <br />
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boil in microwave until agarose beads are dissloved. <br />
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2. Cool to ~ 55 oC, add 5 ul 1000 X GRGreen in water <br />
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3. Pour to tray, wait for 15 to 30 min to solidify. You can also put the gel tray at 4 oC for faster cooling. <br />
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4. Replace buffer if you want to do cloning. <br />
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5. Run gel at 150 or 200 V, 15 to 30 min. <br />
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6. Take a gel picture. Remove gloves before touching the blue light box power switch and camera. <br />
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Note: Never touch camera and memory card using your gloves. $1000 deduction from your salary if you do. <br />
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[[Category:Protocols]]</div>Paul.xu