Paul.xu: Created page with "Aldolase Vp16 MBP fusion expression.jpg Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3)
MW: 10(bottom), ..."

2010-11-05T20:44:03Z

Created page with "400x400px|Aldolase Vp16 MBP fusion expression.jpg Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3) MW: 10(bottom), ..."

New page

[[Image:Aldolase Vp16 MBP fusion expression.jpg|400x400px|Aldolase Vp16 MBP fusion expression.jpg]]

Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3) 

MW: 10(bottom), 17, 28(red), 36, 55, 72(red), 95, 130, 250. 

1, His-SM2 

2, pDB.His.MBP-Aldolase, after purification on Ni and  Maltose columns 

3, FT, flow through, Maltose column 

4, pDB.His.MBP-VP16, uninduced BL21(DE3),  eluate from Ni beads. 

5, pDB.His.MBP-VP16, IPTG induced BL21(DE3), clone 1, eluate from Ni beads. 

6, pDB.His.MBP-VP16, IPTG induced BL21(DE3), clone 2, eluate from Ni beads. 

7, pET28b-VP16, uninduced BL21(DE3), eluate from Ni beads. 

8, pET28b-VP16, IPTG induced BL21(DE3), clone 1, eluate from Ni beads. 

9, pET28b-VP16, IPTG induced BL21(DE3), clone 2, eluate from Ni beads. 

 

[[Image:Vp16-MBP_expression.jpg]] 

Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3)

MW: 10(bottom), 17, 28(red), 36, 55, 72(red), 95, 130, 2 

1, pDB.His.MBP-Aldolase after Ni and Maltose columns 

2, pDB.His.MBP-VP16, flow through after Ni and Maltose columns 

3, pDB.His.MBP-Vp16, after Ni and maltose columns 

4, pDB.His.MBP-Vp16, after Ni and maltose columns 

5, Superfolder GFP

6, Superfolder GFP-TPA fusion (forgot to add protein sample).

7, G-Luciferase

8, Insuin

9, Infared Fluorescent protein.

--[[User:Paul.xu|Paul.xu]] 15:42, 5 November 2010 (EST)

[[Category:Protein_Expression]]

Aldolase-Expression - Revision history

Paul.xu: Created page with "Aldolase Vp16 MBP fusion expression.jpg Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3) MW: 10(bottom), ..."

Paul.xu: Created page with "Aldolase Vp16 MBP fusion expression.jpg Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3)
MW: 10(bottom), ..."