https://eln.excellgen.com/index.php?action=history&feed=atom&title=Amylose_Column_Regeneration 2026-10-18T02:15:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Amylose_Column_Regeneration&diff=9719&oldid=prev 2013-07-12T22:01:56Z

<p></p> <p><b>New page</b></p><div>== Regenerating the Amylose Resin Column&lt;br&gt; ==<br /> <br /> At &#039;&#039;&#039;room temperature&#039;&#039;&#039;: <br /> <br /> &lt;br&gt;1. Water: 3 column volumes&lt;br&gt; <br /> <br /> 2. Wash with saline buffer 20 mM Tris-HCl, 200 mM NaCl and 1 mM EDTA&lt;br&gt;3. 0.1% SDS: 3 column volumes at room temperature&lt;br&gt;4. Water: 1 column volumes&lt;br&gt;5. PBS: 3 column volumes&lt;br&gt; <br /> <br /> 6. Store column&amp;nbsp; in cold room <br /> <br /> Resin can be washed very briefly with 0.1 N NaOH followed by water washing&amp;nbsp; step.&lt;br&gt; <br /> <br /> Alternative method:&lt;br&gt;Regeneration 1: 50 mM HEPES, 4 M Urea, 0.5% w/v SDS pH 7.4.&lt;br&gt;Regeneration 2: 50 mM HEPES, 150 mM (NH4)2SO4, 2 mM EDTA, 2 mM EGTA pH 7.4.&lt;br&gt;Regenerated resin can be stored in 20% ethanol at 4 °C&lt;br&gt;&lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Protocols]]</div>

Paul.xu