https://eln.excellgen.com/index.php?action=history&feed=atom&title=Baculovirus_Cre_FLP_QC_20141009_20141013 2026-10-18T09:55:11Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Baculovirus_Cre_FLP_QC_20141009_20141013&diff=13459&oldid=prev 2014-10-22T16:54:58Z

<p></p> <p><b>New page</b></p><div>VLP from sf9 cells. <br /> <br /> Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration, then ultrafiltrated again, filtered on 10/10/2014&lt;br&gt; <br /> <br /> Original FLP: VLP in sf9 cell culture medium&lt;br&gt; <br /> <br /> Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration , filtered on 10/08/2014&lt;br&gt; <br /> <br /> Final Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration ,then ultrafiltrated again, filtered on 10/10/2014&lt;br&gt; <br /> <br /> Reporter: 2013 for Flp, 2181 for Cre&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Oct. 9 2014 10:00 am&amp;nbsp;: split 293 cells&lt;br&gt; <br /> <br /> Oct. 9 2014 18:00 am Transfect 293 cell (70--80%) using lipofectamin 2000&lt;br&gt; <br /> <br /> Oct.10 2014 10:00 am: 40%-50% cells&amp;nbsp; turns red , cells are in good condition, add Flp and&amp;nbsp; Cre to reporter cells&lt;br&gt; <br /> <br /> {| width=&quot;1050&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> A1: &lt;br&gt; <br /> <br /> 75ul original FLP <br /> <br /> | <br /> A2:&lt;br&gt; <br /> <br /> 0 ul Purified FLP <br /> <br /> | <br /> A3:&lt;br&gt; <br /> <br /> 10ul Final Purified FLP <br /> <br /> | <br /> A4:&lt;br&gt; <br /> <br /> 25ul Final Purified FLP <br /> <br /> | <br /> A5:&lt;br&gt; <br /> <br /> 50ul Final Purified FLP <br /> <br /> | <br /> A6:&lt;br&gt; <br /> <br /> 75ul Final Purified FLP <br /> <br /> |-<br /> | B1: <br /> 100ul Final Purified FLP&lt;br&gt; <br /> <br /> | B2: <br /> 150ul Final Purified FLP&lt;br&gt; <br /> <br /> | B3: <br /> 200ul Final Purified FLP&lt;br&gt; <br /> <br /> | B4: <br /> 50ul Final Purified FLP (unfiltered)&lt;br&gt; <br /> <br /> | B5: <br /> 75ul Final Purified FLP (unfiltered)&lt;br&gt; <br /> <br /> | B6: <br /> 150ul Final Purified FLP (unfiltered)&lt;br&gt; <br /> <br /> |-<br /> | <br /> C1: <br /> <br /> 75ul Purified Cre&lt;br&gt; <br /> <br /> | C2: <br /> 0ul PurifiedCre&lt;br&gt; <br /> <br /> | C3: <br /> 10ul Final Purified Cre&lt;br&gt; <br /> <br /> | C4: <br /> 25ul Final Purified Cre&lt;br&gt; <br /> <br /> | C5: <br /> 50ul Final Purified Cre&lt;br&gt; <br /> <br /> | C6: <br /> 75ul Final Purified Cre&lt;br&gt; <br /> <br /> |-<br /> | D1: <br /> 100 ul Final Purified Cre&lt;br&gt; <br /> <br /> | D2: <br /> 150 ul Final Purified Cre&lt;br&gt; <br /> <br /> | <br /> D3:&lt;br&gt; <br /> <br /> 200 ul Final Purified Cre <br /> <br /> | <br /> D4: <br /> <br /> 50ul Final Purified CRE (unfiltered)&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> D5: <br /> <br /> 75ul Final Purified CRE (unfiltered) <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> D6: <br /> <br /> 150ul Final Purified CRE (unfiltered) <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> |}<br /> <br /> Oct.12 2014&amp;nbsp; take photos <br /> <br /> [[Image:FLP Cre VLP QC 2014 10 12.JPG]] &lt;br&gt;picture of 2014/10/16 <br /> <br /> {| width=&quot;1050&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> A1: &lt;br&gt; <br /> <br /> 75ul original FLP <br /> <br /> | <br /> A2:&lt;br&gt; <br /> <br /> 0 ul Purified FLP <br /> <br /> | <br /> A3:&lt;br&gt; <br /> <br /> 10ul Final Purified FLP <br /> <br /> | <br /> A4:&lt;br&gt; <br /> <br /> 25ul Final Purified FLP <br /> <br /> | <br /> A5:&lt;br&gt; <br /> <br /> 50ul Final Purified FLP <br /> <br /> |-<br /> | <br /> A6:&lt;br&gt; <br /> <br /> 75ul Final Purified FLP <br /> <br /> | B1: <br /> 100ul Final Purified FLP&lt;br&gt; <br /> <br /> | B2: <br /> 150ul Final Purified FLP&lt;br&gt; <br /> <br /> | B3: <br /> 200ul Final Purified FLP&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | <br /> C1: 75ul Purified Cre <br /> <br /> | C2: 0ul PurifiedCre <br /> | C3: 10ul Final Purified Cre <br /> | C4: 25ul Final Purified Cre <br /> | C5: 50ul Final Purified Cre<br /> |-<br /> | C6: 75ul Final Purified Cre <br /> | D1: <br /> 100 ul Final Purified Cre&lt;br&gt; <br /> <br /> | D2: <br /> 150 ul Final Purified Cre&lt;br&gt; <br /> <br /> | <br /> D3:&lt;br&gt; <br /> <br /> 200 ul Final Purified Cre <br /> <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; Unfiltered: all contaminated <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:FLP Cre QC 2014 10 16.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:QC]]</div>

Excellgen