https://eln.excellgen.com/index.php?action=history&feed=atom&title=Baculovirus_Cre_FLP_QC_20141020_20141025 Baculovirus Cre FLP QC 20141020 20141025 - Revision history 2026-10-18T14:43:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Baculovirus_Cre_FLP_QC_20141020_20141025&diff=13513&oldid=prev Excellgen at 21:46, 30 October 2014 2014-10-30T21:46:19Z <p></p> <p><b>New page</b></p><div>VLP from sf9 cells. <br /> <br /> Original FLP: VLP in sf9 cell culture medium&lt;br&gt; <br /> <br /> Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration, then ultrafiltrated again, filtered on 10/10/2014&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration , filtered on 10/08/2014&lt;br&gt; <br /> <br /> Final Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration ,then ultrafiltrated again, filtered on 10/10/2014&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Reporter: 2013 for Flp, 2181 for Cre&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Oct. 20 2014 Morning&amp;nbsp;: split 293 cells&lt;br&gt; <br /> <br /> Oct. 20 2014 afternoon Transfect 293 cell (70--80%) using lipofectamin 2000&lt;br&gt; <br /> <br /> Oct.21 2014 10:00 am: 70%-80% cells&amp;nbsp; turns red , cells are in good condition, add Flp and&amp;nbsp; Cre to reporter cells&lt;br&gt; <br /> <br /> {| width=&quot;1216&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> A1: &lt;br&gt; <br /> <br /> 0ul original FLP <br /> <br /> | <br /> A2:&lt;br&gt; <br /> <br /> 50ul original FLP <br /> <br /> | <br /> A3:&lt;br&gt; <br /> <br /> 100 ul original FLP <br /> <br /> | <br /> A4:&lt;br&gt; <br /> <br /> 0 ul Purified cre <br /> <br /> | <br /> A5:&lt;br&gt; <br /> <br /> 25ul&amp;nbsp; Purified cre <br /> <br /> | <br /> A6:&lt;br&gt; <br /> <br /> 75ul Final Purified FLP <br /> <br /> |-<br /> | B1: <br /> 50ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> | B2: <br /> 100ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> | B3: <br /> 150ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> | B4: <br /> 25 ul Final Purified FLP &lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> | B5: <br /> 50 ul Final Purified FLP &lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> | B6: <br /> 75 ul Final Purified FLP &lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> |-<br /> | <br /> C1: <br /> <br /> 50ul Final Purified FLP <br /> <br /> (filtered on 10/12/2014)&lt;br&gt; <br /> <br /> | C2: <br /> 100ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/12/2014)&lt;br&gt; <br /> <br /> | C3: <br /> 150ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/12/2014)&lt;br&gt;&lt;br&gt; <br /> <br /> | C4: <br /> 25ul Final Purified Cre&lt;br&gt; <br /> <br /> (filtered on 10/12/2014)&lt;br&gt; <br /> <br /> | C5: <br /> 50ul Final Purified Cre&lt;br&gt; <br /> <br /> (filtered on 10/12/2014)&lt;br&gt; <br /> <br /> | C6: <br /> 75ul Final Purified Cre&lt;br&gt; <br /> <br /> (filtered on 10/12/2014)&lt;br&gt; <br /> <br /> |}<br /> <br /> Oct.22 2014&amp;nbsp; 24hours [[Image:20141022cre flp vlpqc.jpg]] <br /> <br /> &amp;nbsp;Oct.23 2014 48hours &lt;br&gt;[[Image:20141023cre flp vlpqc.jpg]]&lt;br&gt; &lt;br&gt; <br /> <br /> Oct242014&lt;br&gt; <br /> <br /> [[Image:20141024cre_flp_vlpqc.jpg]]<br /> <br /> [[Category:QC]]</div> Excellgen