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Baculovirus Cre FLP QC 2014 10 09 - Revision history
2026-10-18T02:19:28Z
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Excellgen at 16:55, 22 October 2014
2014-10-22T16:55:48Z
<p></p>
<p><b>New page</b></p><div>VLP from sf9 cells. <br />
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Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration and purified by gel filtration <br />
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Original FLP: VLP in sf9 cell culture medium<br> <br />
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Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialysis and purified by gel filtration, filtered on 10/08/2014<br> <br />
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Reporter: 2013 for Flp, 2181 for Cre<br> <br />
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<br> <br />
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Oct. 7 2014 17:00 pm&nbsp;: Transfect 293 cell (70--80%) using lipofectamin 2000<br> <br />
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Oct.8 2014 9:00 am: 20%-30% cells&nbsp; turns red , cells are in good condition, add Flp and&nbsp; Cre to reporter cells<br> <br />
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{| width="822" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| <br />
A1: <br> <br />
<br />
25ul Purified FLP <br />
<br />
| <br />
A2:<br> <br />
<br />
50ul Purified FLP <br />
<br />
| <br />
A3:<br> <br />
<br />
75ul Purified FLP <br />
<br />
| <br />
A4:<br> <br />
<br />
150ul Purified FLP <br />
<br />
| <br />
A5:<br> <br />
<br />
75ul Original FLP <br />
<br />
| <br />
A6:<br> <br />
<br />
150ul Original FLP <br />
<br />
|-<br />
| <br />
B1: <br />
<br />
25ul Purified Cre<br> <br />
<br />
| <br />
B2: <br />
<br />
50ul Purified Cre<br> <br />
<br />
| <br />
B3: <br />
<br />
75ul Purified Cre<br> <br />
<br />
| <br />
B4: <br />
<br />
100ul Purified Cre<br> <br />
<br />
| <br />
&nbsp;B5: <br />
<br />
150ul Purified Cre<br> <br />
<br />
| <br />
B6: <br />
<br />
200ul Purified Cre<br> <br />
<br />
|}<br />
<br />
Oct.9 2014 9:00 am take photos <br />
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B4,B5 cell are not vey good <br />
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B6 some cells dies, bad<br> [[Image:CRE FLP 239 LIPO2000.JPG]]<br> <br />
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<br> <br />
<br />
Oct. 7 2014 17:00 pm&nbsp;: Split reporter cells into 24well plate, (2013 from another 24 well plate, 2181 from 10cm plate, transfected on Oct,3)<br> <br />
<br />
Oct.8 2014 9:00 am: Flp reporter cells are red ,but in bad condition, only few Cre reporter cells are red <br />
<br />
add Flp and Cre to reporter cells<br> <br />
<br />
{| width="822" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| <br />
A1: <br> <br />
<br />
0ul Purified FLP <br />
<br />
| <br />
A2:<br> <br />
<br />
25ul Purified FLP <br />
<br />
| <br />
A3:<br> <br />
<br />
50ul Purified FLP <br />
<br />
| <br />
A4:<br> <br />
<br />
75ul Purified FLP <br />
<br />
| <br />
A5:<br> <br />
<br />
100ul Original FLP <br />
<br />
| <br />
A6:<br> <br />
<br />
150ul Original FLP <br />
<br />
|-<br />
| B1: <br />
200ul Purified FLP <br> <br />
<br />
| B2: <br />
50ul Original FLP <br> <br />
<br />
| B3: <br />
75ul Original FLP<br> <br />
<br />
| B4: <br />
100ul Original FLP<br> <br />
<br />
| B5: <br />
100ul Original FLP<br> <br />
<br />
| B6: <br />
150ul FIRST FILTERED FLP<br> <br />
<br />
|-<br />
| <br />
C1: <br />
<br />
10ul Purified Cre<br> <br />
<br />
| C2: <br />
25ul Purified Cre<br> <br />
<br />
| C3: <br />
50ul Purified Cre<br> <br />
<br />
| C4: <br />
75ul Purified Cre<br> <br />
<br />
| C5: <br />
100ul Purified Cre<br> <br />
<br />
| C6: <br />
125ul Purified Cre<br> <br />
<br />
|-<br />
| D1: <br />
150ul Purified Cre<br> <br />
<br />
| D2: <br />
200ul Purified Cre<br> <br />
<br />
| CONTROL<br> <br />
| <br> <br />
| <br> <br />
| <br><br />
|}<br />
<br />
<br> <br> <br />
<br />
[[Image:CRE FLP OLD239.JPG]] <br />
<br />
[[Category:QC]]</div>
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