https://eln.excellgen.com/index.php?action=history&feed=atom&title=Baculovirus_Cre_FLP_vlpQC_cre_QC_20141103 Baculovirus Cre FLP vlpQC cre QC 20141103 - Revision history 2026-10-18T03:33:17Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Baculovirus_Cre_FLP_vlpQC_cre_QC_20141103&diff=13551&oldid=prev Excellgen: Created page with "VLP from sf9 cells. Original FLP: VLP in sf9 cell culture medium<br> Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration..." 2014-11-03T20:04:47Z <p>Created page with &quot;VLP from sf9 cells. Original FLP: VLP in sf9 cell culture medium&lt;br&gt; Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration...&quot;</p> <p><b>New page</b></p><div>VLP from sf9 cells. <br /> <br /> Original FLP: VLP in sf9 cell culture medium&lt;br&gt; <br /> <br /> Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration, then ultrafiltrated again, filtered on 10/10/2014&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration , filtered on 10/08/2014&lt;br&gt; <br /> <br /> Final Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration ,then ultrafiltrated again, filtered on 10/10/2014&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Reporter: 2013 for Flp, 2181 for Cre&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Oct. 30 2014 Morning&amp;nbsp;: split 293 cells&lt;br&gt; <br /> <br /> Oct. 30 2014 afternoon Transfect 293 cell (70--80%) using lipofectamin 2000&lt;br&gt; <br /> <br /> Oct.31 2014 10:00 am: 70%-80% cells&amp;nbsp; turns red , cells are in good condition, add Flp and&amp;nbsp; Cre to reporter cells&lt;br&gt; <br /> <br /> {| width=&quot;1216&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> A1: &lt;br&gt; <br /> <br /> 75ul original FLP <br /> <br /> | <br /> A2:&lt;br&gt; <br /> <br /> 50ul Final Purified FLP<br /> <br /> (filtered on 10/10/2014)<br /> <br /> | &lt;br&gt;<br /> | <br /> A3:&lt;br&gt; <br /> <br /> 75ul Purified cre <br /> <br /> (filtered on 10/8/2014)&lt;br&gt;<br /> <br /> | <br /> A4:&lt;br&gt; <br /> <br /> 50 ul Final Purified FLP<br /> <br /> (filtered on 10/10/2014)<br /> <br /> | &lt;br&gt;<br /> | <br /> A5:&lt;br&gt; <br /> <br /> 1uM cre&lt;br&gt;<br /> <br /> lot#: 20130826&lt;br&gt;<br /> <br /> | <br /> A6:&lt;br&gt; <br /> <br /> 2.5uM cre&lt;br&gt;<br /> <br /> lot#: 20130826&lt;br&gt;<br /> <br /> |-<br /> | B1: <br /> 0ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/24/2014)&lt;br&gt; <br /> <br /> | B2: <br /> 75ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/242014)&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> | B3: <br /> 0ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/29/2014)&lt;br&gt; <br /> <br /> | B4: <br /> 50ul Final Purified FLP &lt;br&gt; <br /> <br /> (filtered on 10/10/2014)&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> | <br /> B5:<br /> <br /> 1uM cre<br /> <br /> lot#: 20131115&lt;br&gt;<br /> <br /> | <br /> B6:<br /> <br /> 2.5uM cre<br /> <br /> lot#: 20131115&lt;br&gt;<br /> <br /> |-<br /> | <br /> C1: <br /> <br /> 100ul Final Purified FLP <br /> <br /> (filtered on 10/24/2014)&lt;br&gt; <br /> <br /> | C2: <br /> 150ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/24/2014)&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> | C3: <br /> 75ul Final Purified FLP&lt;br&gt; <br /> <br /> (filtered on 10/29/2014)&lt;br&gt;&lt;br&gt; <br /> <br /> | C4: <br /> 100ul Final Purified Cre&lt;br&gt; <br /> <br /> (filtered on 10/29/2014)&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> | C5:<br /> 1uM cre<br /> <br /> lot#: 20131219&lt;br&gt; <br /> <br /> | C6:<br /> 2.5uM cre<br /> <br /> lot#: 20131219&lt;br&gt; <br /> <br /> |-<br /> | D1:<br /> 200ul Final Purified FLP<br /> <br /> (filtered on 10/24/2014)&lt;br&gt;<br /> <br /> | D2:<br /> 250ul Final Purified FLP<br /> <br /> (filtered on 10/24/2014)&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> | D3:<br /> 150ul Final Purified FLP<br /> <br /> (filtered on 10/29/2014)&lt;br&gt;&lt;br&gt;<br /> <br /> | D4:<br /> 150ul Final Purified FLP<br /> <br /> (filtered on 10/29/2014)&lt;br&gt;&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> | <br /> D5:<br /> <br /> 1uM cre<br /> <br /> lot#: 56375&lt;br&gt;<br /> <br /> | <br /> D6:<br /> <br /> 2.5uM cre<br /> <br /> lot#: 56375&lt;br&gt;<br /> <br /> |}<br /> <br /> Nov.3 <br /> <br /> [[Image:20141103cre flp vlp creqc.jpg]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:QC]]</div> Excellgen