https://eln.excellgen.com/index.php?action=history&feed=atom&title=CD22CD22 - Revision history2026-10-18T02:35:31ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=CD22&diff=16421&oldid=prevPaul.xu at 20:54, 28 August 20172017-08-28T20:54:29Z<p></p>
<p><b>New page</b></p><div>CD22 was amplified from plasmid pRKm22 using the following oligomers: 22COD 5'GTGAGTGAGAATTCATGCATCTCCTCGGCCCCTG-3' and 33NON 5'-TCACTCACTCGCGGCCGCTTCGCCTGCCGATGGTCTC-3'. pRKm22 is a plasmid encoding full-length human CD22 obtained by cloning from a Daudi cDNA Quick clone library (Clontech). The oligomers introduced EcoRI and NotI restriction sites. After digestion with NotI and EcoRI, the PCR product was purified and cloned into vector pCDNA1.1-Fc between NotI and EcoRI sites creating plasmid pCDNA1.1–22-Fc. The 293T cells were transfected with plasmid pCDNA1.1– 22-Fc by standard CaPO4 precipitation<br />
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Human Siglec-2 (176-687), His Tag (SI2-H5228) is expressed from human 293 cells (HEK293). It contains AA Trp 176 - Arg 687 (Accession # P20273-1). Predicted N-terminus: Trp 176<br />
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Siglec-2(Trp 176 - Arg 687) Poly-his P20273-1<br />
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This protein carries a polyhistidine tag at the C-terminus.<br />
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The protein has a calculated MW of 57.8 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.<br />
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[http://www.uniprot.org/uniprot/P20273 http://www.uniprot.org/uniprot/P20273] 20 – 687 Extracellular 668aa<br />
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DSSKWVFEHPETLYAWEGACVWIPCTYRALDGDLESFILFHNPEYNKNTSKFDGTRLYES TKDGKVPSEQKRVQFLGDKNKNCTLSIHPVHLNDSGQLGLRMESKTEKWMERIHLNVSER PFPPHIQLPPEIQESQEVTLTCLLNFSCYGYPIQLQWLLEGVPMRQAAVTSTSLTIKSVF TRSELKFSPQWSHHGKIVTCQLQDADGKFLSNDTVQLNVKHTPKLEIKVTPSDAIVREGD SVTMTCEVSSSNPEYTTVSWLKDGTSLKKQNTFTLNLREVTKDQSGKYCCQVSNDVGPGR SEEVFLQVQYAPEPSTVQILHSPAVEGSQVEFLCMSLANPLPTNYTWYHNGKEMQGRTEE KVHIPKILPWHAGTYSCVAENILGTGQRGPGAELDVQYPPKKVTTVIQNPMPIREGDTVT LSCNYNSSNPSVTRYEWKPHGAWEEPSLGVLKIQNVGWDNTTIACAACNSWCSWASPVAL NVQYAPRDVRVRKIKPLSEIHSGNSVSLQCDFSSSHPKEVQFFWEKNGRLLGKESQLNFD SISPEDAGSYSCWVNNSIGQTASKAWTLEVLYAPRRLRVSMSPGDQVMEGKSATLTCESD ANPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQGTNSVGKGRSPLSTLTVYY SPETIGRR<br />
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[https://www.ncbi.nlm.nih.gov/nuccore/297374827 https://www.ncbi.nlm.nih.gov/nuccore/297374827]<br />
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ATGCATCTCCTCGGCCCCTGGCTCCTGCTCCTGGTTCTAGAATACTTGGCTTTCTCTGACTCAAGTAAATGGGTTTTTGAGCACCCTGAAACCCTCTACGCCTGGGAGGGGGCCTGCGTCTGGATCCCCTGCACCTACAGAGCCCTAGATGGTGACCTGGAAAGCTTCATCCTGTTCCACAATCCTGAGTATAACAAGAACACCTCGAAGTTTGATGGGACAAGACTCTATGAAAGCACAAAGGATGGGAAGGTTCCTTCTGAGCAGAAAAGGGTGCAATTCCTGGGAGACAAGAATAAGAACTGCACACTGAGTATCCACCCGGTGCACCTCAATGACAGTGGTCAGCTGGGGCTGAGGATGGAGTCCAAGACTGAGAAATGGATGGAACGAATACACCTCAATGTCTCTGAAAGGCCTTTTCCACCTCATATCCAGCTCCCTCCAGAAATTCAAGAGTCCCAGGAAGTCACTCTGACCTGCTTGCTGAATTTCTCCTGCTATGGGTATCCGATCCAATTGCAGTGGCTCCTAGAGGGGGTTCCAATGAGGCAGGCTGCTGTCACCTCGACCTCCTTGACCATCAAGTCTGTCTTCACCCGGAGCGAGCTCAAGTTCTCCCCACAGTGGAGTCACCATGGGAAGATTGTGACCTGCCAGCTTCAGGATGCAGATGGGAAGTTCCTCTCCAATGACACGGTGCAGCTGAACGTGAAGCACACCCCGAAGTTGGAGATCAAGGTCACTCCCAGTGATGCCATAGTGAGGGAGGGGGACTCTGTGACCATGACCTGCGAGGTCAGCAGCAGCAACCCGGAGTACACGACGGTATCCTGGCTCAAGGATGGGACCTCGCTGAAGAAGCAGAATACATTCACGCTAAACCTGCGCGAAGTGACCAAGGACCAGAGTGGGAAGTACTGCTGTCAGGTCTCCAATGACGTGGGCCCGGGAAGGTCGGAAGAAGTGTTCCTGCAAGTGCAGTATGCCCCGGAACCTTCCACGGTTCAGATCCTCCACTCACCGGCTGTGGAGGGAAGTCAAGTCGAGTTTCTTTGCATGTCACTGGCCAATCCTCTTCCAACAAATTACACGTGGTACCACAATGGGAAAGAAATGCAGGGAAGGACAGAGGAGAAAGTCCACATCCCAAAGATCCTCCCCTGGCACGCTGGGACTTATTCCTGTGTGGCAGAAAACATTCTTGGTACTGGACAGAGGGGCCCGGGAGCTGAGCTGGATGTCCAGTATCCTCCCAAGAAGGTGACCACAGTGATTCAAAACCCCATGCCGATTCGAGAAGGAGACACAGTGACCCTTTCCTGTAACTACAATTCCAGTAACCCCAGTGTTACCCGGTATGAATGGAAACCCCATGGCGCCTGGGAGGAGCCATCGCTTGGGGTGCTGAAGATCCAAAACGTTGGCTGGGACAACACAACCATCGCCTGCGCAGCTTGTAATAGTTGGTGCTCGTGGGCCTCCCCTGTCGCCCTGAATGTCCAGTATGCCCCCCGAGACGTGAGGGTCCGGAAAATCAAGCCCCTTTCCGAGATTCACTCTGGAAACTCGGTCAGCCTCCAATGTGACTTCTCAAGCAGCCACCCCAAAGAAGTCCAGTTCTTCTGGGAGAAAAATGGCAGGCTTCTGGGGAAAGAAAGCCAGCTGAATTTTGACTCCATCTCCCCAGAAGATGCTGGGAGTTACAGCTGCTGGGTGAACAACTCCATAGGACAGACAGCGTCCAAGGCCTGGACACTTGAAGTGCTGTATGCACCCAGGAGGCTGCGTGTGTCCATGAGCCCGGGGGACCAAGTGATGGAGGGGAAGAGTGCAACCCTGACCTGTGAGAGCGACGCCAACCCTCCCGTCTCCCACTACACCTGGTTTGACTGGAATAACCAAAGCCTCCCCTACCACAGCCAGAAGCTGAGATTGGAGCCGGTGAAGGTCCAGCACTCGGGTGCCTACTGGTGCCAGGGGACCAACAGTGTGGGCAAGGGCCGTTCGCCTCTCAGCACCCTCACCGTCTACTATAGCCCGGAGACCATCGGCAGGCGA<br />
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GTGGCTGTGGGACTCGGGTCCTGCCTCGCCATCCTCATCCTGGCAATCTGTGGGCTCAAGCTCCAGCGACGTTGGAAGAGGACACAGAGCCAGCAGGGGCTTCAGGAGAATTCCAGCGGCCAGAGCTTCTTTGTGAGGAATAAAAAGAGATGCAGAGTCCTCAGAGATGCAGAGACCTCCCCCGGACTGCGATGA<br />
<pre>atgcatctcctcggcccctggctcctgctcctggttctagaatacttggctttctctgac<br />
M H L L G P W L L L L V L E Y L A F S D <br />
tcaagtaaatgggtttttgagcaccctgaaaccctctacgcctgggagggggcctgcgtc<br />
S S K W V F E H P E T L Y A W E G A C V <br />
tggatcccctgcacctacagagccctagatggtgacctggaaagcttcatcctgttccac<br />
W I P C T Y R A L D G D L E S F I L F H <br />
aatcctgagtataacaagaacacctcgaagtttgatgggacaagactctatgaaagcaca<br />
N P E Y N K N T S K F D G T R L Y E S T <br />
aaggatgggaaggttccttctgagcagaaaagggtgcaattcctgggagacaagaataag<br />
K D G K V P S E Q K R V Q F L G D K N K <br />
aactgcacactgagtatccacccggtgcacctcaatgacagtggtcagctggggctgagg<br />
N C T L S I H P V H L N D S G Q L G L R <br />
atggagtccaagactgagaaatggatggaacgaatacacctcaatgtctctgaaaggcct<br />
M E S K T E K W M E R I H L N V S E R P <br />
tttccacctcatatccagctccctccagaaattcaagagtcccaggaagtcactctgacc<br />
F P P H I Q L P P E I Q E S Q E V T L T <br />
tgcttgctgaatttctcctgctatgggtatccgatccaattgcagtggctcctagagggg<br />
C L L N F S C Y G Y P I Q L Q W L L E G <br />
gttccaatgaggcaggctgctgtcacctcgacctccttgaccatcaagtctgtcttcacc<br />
V P M R Q A A V T S T S L T I K S V F T <br />
cggagcgagctcaagttctccccacagtggagtcaccatgggaagattgtgacctgccag<br />
R S E L K F S P Q W S H H G K I V T C Q <br />
cttcaggatgcagatgggaagttcctctccaatgacacggtgcagctgaacgtgaagcac<br />
L Q D A D G K F L S N D T V Q L N V K H <br />
accccgaagttggagatcaaggtcactcccagtgatgccatagtgagggagggggactct<br />
T P K L E I K V T P S D A I V R E G D S <br />
gtgaccatgacctgcgaggtcagcagcagcaacccggagtacacgacggtatcctggctc<br />
V T M T C E V S S S N P E Y T T V S W L <br />
aaggatgggacctcgctgaagaagcagaatacattcacgctaaacctgcgcgaagtgacc<br />
K D G T S L K K Q N T F T L N L R E V T <br />
aaggaccagagtgggaagtactgctgtcaggtctccaatgacgtgggcccgggaaggtcg<br />
K D Q S G K Y C C Q V S N D V G P G R S <br />
gaagaagtgttcctgcaagtgcagtatgccccggaaccttccacggttcagatcctccac<br />
E E V F L Q V Q Y A P E P S T V Q I L H <br />
tcaccggctgtggagggaagtcaagtcgagtttctttgcatgtcactggccaatcctctt<br />
S P A V E G S Q V E F L C M S L A N P L <br />
ccaacaaattacacgtggtaccacaatgggaaagaaatgcagggaaggacagaggagaaa<br />
P T N Y T W Y H N G K E M Q G R T E E K <br />
gtccacatcccaaagatcctcccctggcacgctgggacttattcctgtgtggcagaaaac<br />
V H I P K I L P W H A G T Y S C V A E N <br />
attcttggtactggacagaggggcccgggagctgagctggatgtccagtatcctcccaag<br />
I L G T G Q R G P G A E L D V Q Y P P K <br />
aaggtgaccacagtgattcaaaaccccatgccgattcgagaaggagacacagtgaccctt<br />
K V T T V I Q N P M P I R E G D T V T L <br />
tcctgtaactacaattccagtaaccccagtgttacccggtatgaatggaaaccccatggc<br />
S C N Y N S S N P S V T R Y E W K P H G <br />
gcctgggaggagccatcgcttggggtgctgaagatccaaaacgttggctgggacaacaca<br />
A W E E P S L G V L K I Q N V G W D N T <br />
accatcgcctgcgcagcttgtaatagttggtgctcgtgggcctcccctgtcgccctgaat<br />
T I A C A A C N S W C S W A S P V A L N <br />
gtccagtatgccccccgagacgtgagggtccggaaaatcaagcccctttccgagattcac<br />
V Q Y A P R D V R V R K I K P L S E I H <br />
tctggaaactcggtcagcctccaatgtgacttctcaagcagccaccccaaagaagtccag<br />
S G N S V S L Q C D F S S S H P K E V Q <br />
ttcttctgggagaaaaatggcaggcttctggggaaagaaagccagctgaattttgactcc<br />
F F W E K N G R L L G K E S Q L N F D S <br />
atctccccagaagatgctgggagttacagctgctgggtgaacaactccataggacagaca<br />
I S P E D A G S Y S C W V N N S I G Q T <br />
gcgtccaaggcctggacacttgaagtgctgtatgcacccaggaggctgcgtgtgtccatg<br />
A S K A W T L E V L Y A P R R L R V S M <br />
agcccgggggaccaagtgatggaggggaagagtgcaaccctgacctgtgagagcgacgcc<br />
S P G D Q V M E G K S A T L T C E S D A <br />
aaccctcccgtctcccactacacctggtttgactggaataaccaaagcctcccctaccac<br />
N P P V S H Y T W F D W N N Q S L P Y H <br />
agccagaagctgagattggagccggtgaaggtccagcactcgggtgcctactggtgccag<br />
S Q K L R L E P V K V Q H S G A Y W C Q <br />
gggaccaacagtgtgggcaagggccgttcgcctctcagcaccctcaccgtctactatagc<br />
G T N S V G K G R S P L S T L T V Y Y S <br />
ccggagaccatcggcaggcga<br />
P E T I G R R <br />
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gtggctgtgggactcgggtcctgcctcgccatcctcatc<br />
V A V G L G S C L A I L I <br />
ctggcaatctgtgggctcaagctccagcgacgttggaagaggacacagagccagcagggg<br />
L A I C G L K L Q R R W K R T Q S Q Q G <br />
cttcaggagaattccagcggccagagcttctttgtgaggaataaaaagagatgcagagtc<br />
L Q E N S S G Q S F F V R N K K R C R V <br />
ctcagagatgcagagacctcccccggactgcgatga<br />
L R D A E T S P G L R - <br />
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688 – 706 Transmembrane Helical 19aa 707 – 847 Cytoplasmic 141aa<br />
[[Category:Antibodies]]</div>Paul.xu