https://eln.excellgen.com/index.php?action=history&feed=atom&title=CETP_VLP_Expression_Screening 2026-10-18T07:56:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=CETP_VLP_Expression_Screening&diff=20037&oldid=prev 2019-07-12T14:43:06Z

<p></p> <p><b>New page</b></p><div>expression Screening&amp;nbsp; in urea soluble fraction (inclusion body)<br /> <br /> [[File:71119VLPscreening.jpg|RTENOTITLE]]<br /> <br /> {| class=&quot;wikitable&quot;<br /> |-<br /> ! Lane<br /> ! ID<br /> ! Name<br /> ! Host<br /> ! pI/MW<br /> ! Induction Condition<br /> ! Expressed?<br /> |-<br /> | M<br /> | Protein Marker<br /> | PageRuler Plus<br /> | 10 15 25red 35 55 70red 100 130 250<br /> | <br /> | <br /> | <br /> |-<br /> | 1<br /> | 2557<br /> | pET28a-Qbeta-CETP-N1-Avitag-6His<br /> | Nico(DE3)pLysN<br /> | 7.86 / 19.6<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 2<br /> | 2558<br /> | pET28a-Qbeta-CETP-N2-Avitag-6His<br /> | Nico(DE3)pLysN<br /> | 6.43 / 20.2<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 3<br /> | 2559<br /> | pET28a-Qbeta-CETP-N3-Avitag-6His<br /> | Nico(DE3)pLysN<br /> | 7.13 / 20.2<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 4<br /> | 2555<br /> | pET28a-Qbeta-CETP-N13-Avitag-6His<br /> | Nico(DE3)pLysN<br /> | 7.87 / 22.4<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 5<br /> | 2556<br /> | pET28a-Qbeta-CETP-N12-Avitag-6His<br /> | Nico(DE3)pLysN<br /> | 6.75 / 22.6<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 6<br /> | 2560<br /> | pET28a-CETP-N1-AP205<br /> | Nico(DE3)pLysN<br /> | 8.98 / 17.8<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 7<br /> | 2561<br /> | pET28a-CETP-N2-AP205<br /> | Nico(DE3)pLysN<br /> | 7.06 / 18.3<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 8<br /> | 2562<br /> | pET28a-CETP-N3-AP205<br /> | Nico(DE3)pLysN<br /> | 8.65 / 18.3<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 9<br /> | 2563<br /> | pET28a-CETP-N12-AP205<br /> | Nico(DE3)pLysN<br /> | 7.85 / 20.6<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 10<br /> | 2564<br /> | pET28a-CETP-N13-AP205<br /> | Nico(DE3)pLysN<br /> | 8.95 / 20.6<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 11<br /> | 971<br /> | pET28a-SpyCatcher-AP205cp3<br /> | Nico(DE3)pLysN<br /> | 6.50 / 26<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 12<br /> | 2565<br /> | pET29b-MT-MMLV<br /> | Nico(DE3)pLysN<br /> | 9.08 / 79.6<br /> | 37 oC for 4 hours<br /> | yes, more&amp;nbsp; as inclusion body<br /> |-<br /> | 13<br /> | 2566<br /> | pET29a-Qbeta-SpyCatcher-Avitag-6His<br /> | Nico(DE3)pLysN<br /> | 6.20 / 27.7<br /> | 37 oC for 4 hours<br /> | yes, IB<br /> |-<br /> | 14<br /> | 2567<br /> | pET29b-RT-Pol<br /> | Nico(DE3)pLysN<br /> | 6.80 / 101.5<br /> | 37 oC for 4 hours<br /> | yes, not much<br /> |-<br /> | <br /> |}<br /> [[Category:QC]][[Category:Protein Expression]]</div>

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