Paul.xu at 20:09, 20 June 2014

2014-06-20T20:09:49Z

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<blockquote>'''Simple mixing method:''' '''''Example:''' For MPG mediated gene delivery,'' ''peptide carrier/DNA complexes were formed in DMEM or phosphate-buffered saline (500 ml of DMEM containing 100 ng of DNA complexed with MPG at a charge ratio of 5:1) and incubated for 30 min at 37°C. Cells grown'' ''to 60% confluence were then overlaid with these preformed complexes.'' </blockquote>
CPPs pack oligonucleotides into nanocomplexes, which do not

dissociate in the presence of serum proteins and transfect cells more

efficiently than commercially available cationic lipids.

 

Many novel CPPs have been shown to transfect and trigger specific

biological response in a wide range of cell types, including challenging primary and suspension cells without being cytotoxic. In vivo experiments have revealed the capacity of CPP-mediated ONs to effectively inhibit tumor growth in tumor-bearing mice without triggering immune response, emphasizing even more the high therapeutic potential of CPP–ON

complexes.

 

'''Table: Application and effects of CPP-mediated siRNA'''

'''delivery by using noncovalent strategy'''

 

 

{| cellspacing="0" cellpadding="0" title="Cell Penetrating Peptide Synthesis Services"
|-
| width="242" valign="bottom" |
'''CPP'''

| width="92" valign="bottom" |
'''Cells/Model'''

| width="179" valign="bottom" |
'''Effect'''

| width="175" valign="bottom" |
'''Reference'''

|-
| width="242" |
'''MPG''' ac-GALFLGFLGAAGSTMGAWSQPKKKRKV-cys

''The cysteamide stabilises the carrier/DNA particle, as''

''similarly reported for gene delivery mediated by cysteine-containing'' ''peptides.''

 

| width="92" valign="top" |
HeLa and Cos-7 HS68, NIH-3T3

| width="179" valign="top" | Cellular uptake of single- and
double-stranded ONs 50 nmol/l siRNA→85% and 78% luciferase downregulation

| width="175" valign="top" |
Simeoni et al.

|-
| width="242" |
 

| width="92" |
HS68

| width="179" | 100 nmol/l siRNA→60% GAPDH downregulation
| width="175" |
Simeoni et al.

|-
| width="242" |
'''MPGΔNLS''' ac-GALFLGFLGAAGSTMGAWSQPKSKRKV-cys

| width="92" |
HeLa and Cos-7

| width="179" | 50 nmol/l siRNA→95% and 90% luciferase 
downregulation

| width="175" |
Simeoni et al.

|-
| width="242" |
 

| width="92" valign="top" |
 

| width="179" valign="top" |  
| width="175" valign="top" |
 

|-
| width="242" |
'''Stearyl-R8''' 

st-RRRRRRRR-NH2 ''Polyarginine peptides, which are very efficient CPPs have'' ''been used for siRNA delivery into cells.''

 ''Stearylated octaarginine (Stearyl-R8), are able to''

''encapsulate and shuttle nucleic acids through cell membranes without the need to covalently attach the nucleic acid to the'' ''arginine-containing carrier peptide. ''

 

| width="92" |
EGFP-expressing hippocampal neurons

| width="179" | 16 nmol/l siRNA→>50% reduction in EGFP activity
| width="175" |
Tönges et al.

|-
|  
|  
|  
|  
|-
| width="242" |
'''EB1''' LIKLWSHLIHIWFQNRRLKWKKK-amide

| width="92" |
HeLa, HepG2

| width="179" | Luciferase downregulation
| width="175" |
Lundberg et al.

|-
|  
|  
|  
|  
|-
| width="242" |
'''Tat-DRBD''' GRKKRRQRRRPQ-DRBD

| width="92" valign="top" |
H1299, HUVEC, Jurkat T, hESC

| width="179" valign="top" | >90% reduction in GAPDH mRNA level
| width="175" valign="top" |
Eguchi et al.

|-
|  
|  
|  
|  
|-
| width="242" |
'''PF6''' St-AGYLLGK[kk2sa4qn4]INLKALAALAKKIL-NH2

| width="92" |
Hepatoma, MEF, HUVEC, mESC

| width="179" | Significant downregulation of HPRT1
| width="175" |
El-Andaloussi et al.

|}

[[Category:Transfection]][[Category:Paul]]

CPP - Revision history

Paul.xu at 20:09, 20 June 2014