Chromatography Basic - Revision history

Paul.xu: Created page with "=== linear flow (cm/h) to volumetric flow rate (ml/min)
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Volumetric flow rate (ml/min) = Linear flow (cm/h) × column cross-sectional area (cm2)/60=Y * Pi * d^{2
2012-11-14T14:02:04Z
Created page with "=== linear flow (cm/h) to volumetric flow rate (ml/min)<br> === <br>Volumetric flow rate (ml/min) = Linear flow (cm/h) × column cross-sectional area (cm2)/60=Y * Pi * d<sup>2</..."
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=== linear flow (cm/h) to volumetric flow rate (ml/min)<br> ===

<br>Volumetric flow rate (ml/min) = Linear flow (cm/h) × column cross-sectional area (cm2)/60=Y * Pi * d<sup>2</sup> /604<br>

Y = linear flow in cm/h<br>d = column inner diameter in cm<br>''Example:''<br>What is the volumetric flow rate in an XK 16/70 column (i.d. 1.6 cm) when the linear flow is 150 cm/h?<br>Y = linear flow = 150 cm/h<br>d = inner diameter of the column = 1.6 cm<br>Volumetric flow rate = 150 Pi * 1.6 * 1.6 ml/min / 60 * 4 = 5.03 ml/min<br>

For XK16 column, 60 cm/h =2 ml/min<br>

For Hitrap 1 ml column: ID=0.7 cm,  60 cm/h =0.4 ml/min<br>

For HiTrap 5 ml column and XK16 column: ID=1.6  cm,  60 cm/h =2 ml/min

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