https://eln.excellgen.com/index.php?action=history&feed=atom&title=Circular_Assembly_Amplification 2026-10-18T13:02:34Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Circular_Assembly_Amplification&diff=18920&oldid=prev 2018-08-05T16:53:44Z

<p>Created page with &quot;Design plus  and  minus strand oligonucleotides  in such a way that, upon annealing, complementary oligos would overlap as a circular structure, leaving no gap...&quot;</p> <p><b>New page</b></p><div>Design plus&amp;nbsp; and&amp;nbsp; minus strand oligonucleotides&amp;nbsp; in such a way that, upon annealing, complementary oligos would overlap as a circular structure, leaving no gap to fill.&amp;nbsp;<br /> <br /> Design one more guiding oligo (24th minus strand)&amp;nbsp;&amp;nbsp;to join 5’ and 3’ ends of the&amp;nbsp; target sequence. <br /> <br /> The length of each oligo is approximately 40 to 50 base pairs, and the overlap of each oligo should be designed to have a melting temperature of 60ºC by the nearestneighbor method.&amp;nbsp;<br /> <br /> &lt;span style=&quot;font-family:courier new,courier,monospace;&quot;&gt;ATG ATC GTT CTG TTC GTT GAC TTC GAC TAC TTC TAC GCT CAG ~ GTT GAA GAA GTT CTG AAC CCG TCT CTG AAA GGT AAA CCG GTT G&lt;/span&gt;&lt;span style=&quot;font-family:courier new,courier,monospace;&quot;&gt;&lt;/span&gt;&lt;span style=&quot;font-family:courier new,courier,monospace;&quot;&gt;&lt;/span&gt;&lt;span style=&quot;font-family:courier new,courier,monospace;&quot;&gt;&lt;/span&gt;&lt;span style=&quot;font-family:courier new,courier,monospace;&quot;&gt;&lt;/span&gt;&amp;nbsp; &amp;nbsp; &amp;nbsp;</div>

Paul.xu