https://eln.excellgen.com/index.php?action=history&feed=atom&title=Cloning_of_alpaca_VHH_cDNACloning of alpaca VHH cDNA - Revision history2026-10-18T03:30:09ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Cloning_of_alpaca_VHH_cDNA&diff=18606&oldid=prevPaul.xu at 20:26, 8 July 20182018-07-08T20:26:00Z<p></p>
<p><b>New page</b></p><div>RT primers:&nbsp;Camelus,&nbsp;Lama<br />
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ATGGAGAGGACGTCCTTGGGT<br />
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TTCGGGGGGAAGAYRAAGAC<br />
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PCR<br />
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AlpVh-L (GGTGGTCCTGGCTGC);&nbsp;<br/>AlpVh-F1 (GATCGCCGGCCAGKTGCAGCTCGTGGAGTCNGGNGG);&nbsp;<br/>AlpVHH-R1 (GATCACTAGTGGGGTCTTCGCTGTGGTGCG)&nbsp;:&nbsp; primes within the short hinge coding region at the same site as Lam07;&nbsp;<br/>and AlpVHH-R2 (GATCACTAGTTTGTGGTTTTGGTGTCTTGGG)<br />
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[[Category:Antibodies]]<br />
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use&nbsp;E. coli Rosetta-gami for VHH antibody expression</div>Paul.xu