https://eln.excellgen.com/index.php?action=history&feed=atom&title=Cloning_report
Cloning report - Revision history
2026-10-18T02:14:55Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=Cloning_report&diff=18732&oldid=prev
Crm at 18:47, 17 July 2018
2018-07-17T18:47:15Z
<p></p>
<p><b>New page</b></p><div>{| border="1" cellspacing="1" cellpadding="1" style="width: 879px;"<br />
|-<br />
| '''ID'''<br />
| '''Status'''<br />
| '''Name'''<br />
| '''Progress'''<br/><br />
| style="width: 464px;" | '''Note'''<br />
|-<br />
| 812<br/><br />
| <br/><br />
| pET30b-phoA<br/><br />
| <br/><br />
| style="width: 464px;" | <br/><br />
|-<br />
| 931<br />
| <br/><br />
| PAK16D ligase<br />
| <br />
pJet1.2<br />
<br />
fragment 2 is good<br />
<br />
fragment 1 is also good<br />
<br />
<span style="color:#FF0000;">need to do digestions</span><br />
<br />
<span style="color:#FF0000;">seqeucning result: junk, E coli chromesome DNA</span><br />
<br />
| style="width: 464px;" | <br/><br />
|-<br />
| 988<br/><br />
| <br/><br />
| 987 pCMVS-LAMP-1_CD133<br/><br />
| -<br />
| style="width: 464px;" | <br/><br />
|-<br />
| 998<br/><br />
| <br/><br />
| pT7A3-6His-NLS-dCas9-NLS-6xHis<br/><br />
| '''6/15/18''':bands on gel seems good but use wrong template because of data base,right one should be 978 not 979<br/><br />
| style="width: 464px;" | <br/><br />
|-<br />
| 999<br/><br />
| <br/><br />
| pT7A30-SssI<br/><br />
| based on 1916 gene synthesis<br />
| style="width: 464px;" | <br/><br />
|-<br />
| 1000<br />
| <br/><br />
| pT7A3-6His-NLS-FnCpf1-NLS-6xHis<br/><br />
| 3' is good<br />
| style="width: 464px;" | <br/><br />
|-<br />
| 3218<br/><br />
| <br/><br />
| pT7A30-ULP1<br/><br />
| PRC from&nbsp;2209 ZM552-ULP1<br />
| style="width: 464px;" | pickup<br />
|-<br />
| <span style="color:#FF0000;">3204</span><br/><br />
| <br/><br />
| pCMV5-OX40-Avi-6H-BirA<br/><br />
| from 3203 pANT7_cGST-TNFRSF4<br/><br />
| style="width: 464px;" | subclone fnished,vector3237<br/><br />
|-<br />
| 3195<br/><br />
| <br/><br />
| pEF1-HTLV-LAMP-1-BCMA<br/><br />
| <br/><br />
| style="width: 464px;" | doing 3206 vector now<br />
|-<br />
| <span style="color:#FF0000;">3225</span><br />
| <br/><br />
| pT7A3-GST-MICA<br/><br />
| <br/><br />
| style="width: 464px;" | subclone fnished<br />
|-<br />
| 3215<br />
| <br/><br />
| pCMV5-MICA-a-Avi-6H-BirA<br/><br />
| <br/><br />
| style="width: 464px;" | subclone finished<br/><div><br/></div><br />
|-<br />
| 3208<br/><br />
| <br/><br />
| pEPD-FW<br/><br />
| <br/><br />
| style="width: 464px;" | sequencing finished<br />
|-<br />
| 941<br />
| <br/><br />
| pT7A3-pMBP-T7-Endo-1<br/><br />
| <br/><br />
| style="width: 464px;" | pickup on 6/29/2018<br />
|-<br />
| 3234<br />
| <br/><br />
| pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP<br/><br />
| <br/><br />
| style="width: 464px;" | sequence finished<br />
|-<br />
| 3235<br/><br />
| <br/><br />
| pCMV5-IL2-MICA-(3c)-Fc-WPRE-OriP<br/><br />
| <br/><br />
| style="width: 464px;" | <br/><br />
|-<br />
| 3234<br/><br />
| <br/><br />
| pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP<br/><br />
| <br/><br />
| style="width: 464px;" | <br/><br />
|-<br />
| 3233&nbsp;<br/><br />
| <br/><br />
| pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP<br/><br />
| good, need to make glycerol stock<br />
| style="width: 464px;" | <br/><br />
|-<br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">3232</span><br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| style="width: 464px;" | <br/><br />
|-<br />
| &nbsp;<br />
| <br/><br />
| <br/><br />
| <br/><br />
| style="width: 464px;" | <br/><br />
|}<br />
[[Category:To Do]] [[Category:My ELN]] [[Category:Tasks]]</div>
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