Crm at 22:41, 21 August 2019

2019-08-21T22:41:53Z

New page

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| Excellgen Cat #
| Clone #
| Size
| Price
| Name
| Supplier Cat #
| style="width: 99px;" | Supplier
| style="width: 58px;" | Lot 
| style="width: 58px;" | Concentration
| Size
| style="width: 285px;" | Storage Buffer
| style="width: 50px;" | Price
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| EG-150
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| 100units, 10U/ul, 10ul
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| EG-151 
| 
| 2,000 U/ml,50ul, 100 units
| $85
| PolyU polymerase,   Schizosaccharomyces pombe 
| M0337S
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 0.34 mg/ml
| 2000U/ml, 30 ul, 60 units
| style="width: 285px;" | 
| style="width: 50px;" | $85
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| EG-1057 
| 1942 
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100,000 U/ml

100U/ul

1ug/ul, 100 ul, 100ug

| $350
| phi29 (φ29) DNA Polymerase 
| EP0094 
| style="width: 99px;" | Thermofisher
| style="width: 58px;" | 
| style="width: 58px;" | 0.1ug/ul, 10u/ul, 
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5000U

500 ul -->

50 ug

50 ul of 1 mg/ml stock + 450 ul buffer

| 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycero
| $826
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| E coli polyA polymeras
| M0276S
| style="width: 99px;" | NEB
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| 20ul, 7.43 mg/ml by reading but has 0.1% Triton X-100
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| T4 DNA ligase , 400units/ul
| M0202L
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 
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1.1mg/ml

0.76mg/ml (1271711)

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| T4 DNA ligase. 2U/ul
| F93301
| style="width: 99px;" | Lucigen
| style="width: 58px;" | 
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| 2.7mg/ml
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| T4 DNA ligase. 2U/ul 
| 46-0117
| style="width: 99px;" | invitrogen
| style="width: 58px;" | 
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| 0.61mg/ml
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| T4 DNA ligase. 5U/ul 
| K-1232
| style="width: 99px;" | Thermofisher
| style="width: 58px;" | 00680547
| style="width: 58px;" | 
| 1.03mg/ml
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| Tag Ligase, 40,000U/ml
| M0208S
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 
| 0.3/mg/ml
|  50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 µg/ml BSA and 50% glycerol. 
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| Bst polymerase, large fragment
| M0275S
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 8000U/ml
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2.32mg/ml

not accurate because Tx-100

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storage buffer has Triton X-100

10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% Triton® X-100 pH 7.1 @ 25°C

based on NEB's specific Activity: 120,000 units/mg,  100U/ul = 20,000U/200ul, 90% purity, then need 200 ug protein in 200 ul., take 10 ul of 29mg/ml-->200 ul storage buffer, protein conc is 1.7 mg/ml

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| T3
| M0317S
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 3000U/ul
| 2mg/ml
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| EG-160
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| T4 PDG
| M0308S
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 10U/ul
| 3.93mg/ml
| storage buffer has 0.15% Triton X-100, do not know exact protein conc
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| EG-1005
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| APE 1
| M0282S
| style="width: 99px;" | NEB
| style="width: 58px;" | 
| style="width: 58px;" | 10U/ul
| 1.24
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[[Category:Cloning]]

Commercial Enzymes - Revision history

Crm at 22:41, 21 August 2019