Crm at 22:42, 2 July 2018

2018-07-02T22:42:46Z

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= Need to sequence  =

'''=====finished but have not sequenced yet======'''

{| border="1" cellspacing="1" cellpadding="1" style="width: 815px;"
|-
| #
| Name 
| Status
| Date
| picture
| Vector#
| style="width: 79px;" | Enzymes
| style="width: 58px;" | Size
| style="width: 77px;" | Antibiotics
| style="width: 41px;" |    Method
| Note
| Next Action
| style="width: 147px;" | QC_sequence clone#
|-
| 2509
| pT7A30-pMBP-Cid 
| 
| 
| [http://www.excellgen.net/eln/index.php/2509_pT7A30-pMBP-Cid result]
| 
| style="width: 79px;" | Nde1 -Xho1 
| style="width: 58px;" | 978
| style="width: 77px;" | K
| style="width: 41px;" | Subclone
| compared with +
| re-doing
| style="width: 147px;" | 
|-
| 3204
| 
| 
| 
| 
| 
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | 
| style="width: 41px;" | 
| 
| done
| style="width: 147px;" | 
|-
| 3210
| pT7A30-Sumo-Ov-ASP1-BCMA 
| 
| 
| [http://www.excellgen.net/eln/index.php/3210_pT7A30-Sumo-Ov-ASP1-BCMA result]
| 1801
| style="width: 79px;" | Nde1-EcoR1 
| style="width: 58px;" | 999
| style="width: 77px;" | K
| style="width: 41px;" | Subclone
| compared with +
| sequencing T7 Term;done 
| style="width: 147px;" | 
|-
| 3215
| pCMV5-MICA-a-Avi-6H-BirA 
| 
| 
| [http://www.excellgen.net/eln/index.php/3215_pCMV5-MICA-a-Avi-6H-BirA#Cloning result]
| 3237
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | Amp
| style="width: 41px;" | subclone
| compared with +and 3237 Vector digestion 
| done
| style="width: 147px;" | 
|-
| 3199
| pT7A3-GST-Avitag 
| 
| 
| [http://www.excellgen.net/eln/index.php/3199_pCMV5-BCMA-Avi-6H-BirA result]
| 3237
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | Amp
| style="width: 41px;" | subclone
| compared with +and 3237 Vector digestion 
| done
| style="width: 147px;" | 
|-
| 3196
| pCMV5-CD47-Avi-6H-BirA 
| 
| 
| [http://www.excellgen.net/eln/index.php/3196_pCMV5-CD47-Avi-6H-BirA result]
| 3237
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | Amp
| style="width: 41px;" | subclone
| compared with +and 3237 Vector digestion 
| done
| style="width: 147px;" | 
|-
| 941
| pT7A3-pMBP-T7-Endo-1 
| 
| 
| [http://www.excellgen.net/eln/index.php/941_pT7A3-pMBP-T7-Endo-1 result]
| 947
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | K
| style="width: 41px;" | subclone
| comnpare with +
| done
| style="width: 147px;" | 
|-
| 129
| 
| 
| 
| 
| 
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | 
| style="width: 41px;" | 
| 
| 
| style="width: 147px;" | 
|-
| 994
| 
| 
| 
| 
| 
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | 
| style="width: 41px;" | 
| 
| 
| style="width: 147px;" | 
|-
| 3197
| 
| 
| 
| 
| 
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | 
| style="width: 41px;" | 
| 
| 
| style="width: 147px;" | 
|-
| 995
| 
| 
| 
| 
| 
| style="width: 79px;" | 
| style="width: 58px;" | 
| style="width: 77px;" | 
| style="width: 41px;" | 
| 
| 
| style="width: 147px;" | 
|}

= Completed and sequenced  =

'''==========completed and sequenced=============='''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| '''ID'''
| '''Status'''
| '''Date'''
| '''Name'''
| '''Progress''' 
| '''Date'''
| Supervisor's Note
| Clone #
| Status
|-
| 3127 
| 
| 
| pET28b-qBeta-qBeta-6XHis 
| PCR qbeta and clone back into 3097
| already finished on 5/3/2018,sequenced 
| glycerol stock ? expression screening?
| 
| GS made
|-
| 938
| 
| 
| PNGase F,
| pJet1.2, sequencing is good, need to subclone into pET30b
| finished
| glycerol stock ? expression screening? 
| 
| GS made
|-
| 939 pET30b-Tte-ExoIII
| 
| 
| Tte-Exo III
|
pJet1.2

sequencing is good, need to subclone into pET30b

| finished
| glycerol stock ? expression screening? 
| 
| GS made
|-
| 942 pET28b-pMBP-VV
| 
| 
| VVN endonuclease
|
already in pJet1.2

need to check by digestion

| finished
| glycerol stock ? expression screening? 
| 
|
GS

made

|-
| 3122
| 
| 
| Crm197, secreted
| already in pJet1.2,subclone to pET30b(vector#500), alrady finished
| [http://www.excellgen.net/eln/index.php/3122_pET30b-CRM197 done],finished
| glycerol stock ? expression screening?
| 
|
GS

made

|-
| 945 
| 
| 
| pT7A30-pMBP 
|
PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL

sequencing is good, completed

| 
| sequencing verfied
| 
|
GS 

made

|-
| 947 
| 
| 
| pT7A30-6His-pMBP 
|
PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL (as template)

sequencing is good, completed

| 
| sequencing verfied 
| 
|
GS

made

|-
| 944
| 
| 
| pET30b-pfu-exo- 
| 944 pET30b-pfu-exo digestion , sequencing result: finished
| 
| glycerol stock ? expression screening? 
| 
|
GS

made

|-
| 824 
| 0
| 
| pET28b-VVTopo 
|
subclone from 832 pMiniT-VVTopo using Nde1 + Xho1

in pET28 vector, sequenced

| 
| glycerol stock ? expression screening? 
| 
|
GS

made

|-
| 993 
| 
| 
| pCold-GST-M13-Gene8 
| +993pCold-GST-M13-Gene8digestion
| 
| sequencing with T7 Term,  expression screening? 
| 
| 
|-
| 992 
| 
| 
| pCold-GST-V5 
| +992pCold-GST-V5digestion
| 
| sequencing with T7 Term, then expression screening
| 
| 
|-
| 991  
| 
| 
| pCold-GST-Flag 
| +991pCold-GST-Flagdigestion
| 
| sequencing with T7 Term, then expression screening 
| 
| 
|-
| 990 
| 
| 
| pCold-GST-HA 
| +990pCold-GST-HAdigestion
| 
| sequencing with T7 Term, then expression screening 
| 
| 
|-
| 989  
| 
| 
| pCold-GST-Myc 
| +989pCold-GST-Mycdigestion
| 
| sequencing with T7 Term, then expression screening 
| 
| 
|-
| 985  
| 
| 
| pCold-GST-3C-AP205-M13-g3p 
| 985pCold-GST-3C-AP205-M13-g3pdigestion, good sequenced, 
| 
| expression screening 
| 
| 
|-
| 997
| 
| 
| pT7A3-Qbeta 
| 997pT7A3-QbetaDigestion , sequenced , good, completed
| 
| no his tag, do not need to do expression screening
| 
| 
|-
| 986
| 
| 
| pT7A3-6His-NLS-Cas9-NLS-6xHis 
| 986pT7A3-6His-NLS-Cas9-NLS-6xHis digestion looks good, good, sequenced
| 
| expression screening? 
| 
|
GS

made

|-
| <div>984</div><div> </div><div> </div><div> </div>
|
| 
| pT7A3-6H-SBP-SUMO-3C
| 6/6/18 sequencing verified 6/5/2018 sequenced 
| 
| expression screening? 
| 
|
GS

made

|-
| 3192
| 
| 
| pCMV5-PD-1-Avi-6H-BirA 
| sequencing good, completed
| 
| make miniprep from 15 ml
| 
|
GS

made

|-
| 3166  
| 
| 
| pCMV-mIgG2b-HC 
| sequencing good, completed 
| 
| make miniprep from 15 ml 
| 
|
GS

made

|-
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{| border="1" cellspacing="1" cellpadding="1" style="width: 759px;"
|-
| #
| Name 
| Status
| Date
| style="width: 37px;" | picture
| style="width: 91px;" | Vector#
| Enzymes
| Size
| Antibiotics
| Method
| Note
| Next Action
| QC_sequence clone#
| Final Status
|-
| 3233
| pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP 
| 
| 
| style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3233_pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP result]
| style="width: 91px;" | 1393
| Nhe1-KpnI 
| 375
| Amp
| Subclone
| compared with +
| sequencing good, completed 
| #1
|
GS

made

|-
| 2503
| pCold-GST-VVTopo 
| 
| 
| style="width: 37px;" | [http://www.excellgen.net/eln/index.php/2503_pCold-GST-VVTopo result]
| style="width: 91px;" | 3161
| Nde1 -Xho1 
| 951
| K
| Subclone
| compared with +
| sequencing good, completed 
| #4
|
GS

made

|-
| 3194
| pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP 
| 
| 
| style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3194_pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP result]
| style="width: 91px;" | 1393
| Nhe1-KpnI 
| 174bp
| Amp
| PCR
| 
| sequencing good, completed 
| #2
|
GS

made

|-
| 3215
| pCMV5-MICA-a-Avi-6H-BirA 
| 
| 
| style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3204_pCMV5-OX40-Avi-6H-BirA#Results result]
| style="width: 91px;" | 3192
| Nhe1-Kpn1
| 285bp
| Amp
| PCR
| 
| sequencing insert is good, subclone to 3232 
| #1
| sub
|-
| 3204
| pCMV5-OX40-Avi-6H-BirA 
| 
| 
| style="width: 37px;" | [http://www.excellgen.net/eln/index.php/3215_pCMV5-MICA-a-Avi-6H-BirA#Cloning result]
| style="width: 91px;" | 1393
| Nhe1+kpn1
| 564bp
| Amp
| PCR
| 
| sequencing insert is good, subclone to 3232
| #5
| sub
|-
| 3191
| pT7A3-GST-Avitag 
| 
| 
| style="width: 37px;" | 
| style="width: 91px;" | 1851
| 
| 
| K
| Annealing
| 
| sequencing good, completed 
| 
| GS Made
|-
| 3232
| pCMV5-Avi-6H-BirA 
| 
| 
| style="width: 37px;" | 
| style="width: 91px;" | 
| 
| 
| 
| 
| 
| 
| 
| 
|}
[[Category:To Do]]

Completed Clones - Revision history

Crm at 22:42, 2 July 2018