https://eln.excellgen.com/index.php?action=history&feed=atom&title=Cre_purification2014_10_27 2026-10-18T02:15:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Cre_purification2014_10_27&diff=13515&oldid=prev 2014-10-30T21:53:50Z

<p>Created page with &quot;2014/10/27&lt;br&gt; &lt;br&gt; lane1-5 : 1L 1708 pT7A2-HTNCre Nico21(DE30plysN&lt;br&gt;                 with...&quot;</p> <p><b>New page</b></p><div>2014/10/27&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> lane1-5&amp;nbsp;: 1L 1708 pT7A2-HTNCre Nico21(DE30plysN&lt;br&gt; <br /> <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; without antiform,&lt;br&gt; <br /> <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; when OD600&amp;gt;1.2, use o.5mM IPTG RT induce overnight, elute from 5ml HisTrap HP&lt;br&gt; <br /> <br /> lane6-9:&amp;nbsp; 1L 1708 pT7A2-HTNCre Nico21(DE30plysN&lt;br&gt; <br /> <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; with antiform and 5020,&lt;br&gt; <br /> <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; when OD600&amp;gt;1.2, use o.5mM IPTG RT induce overnight, elute from 5ml HisTrap HP&lt;br&gt; <br /> <br /> lane 10: 2048 MBP-HCMVUL98(before 3c digestion)&lt;br&gt; <br /> <br /> lane 11: 2048 MBP-HCMVUL98(after 3c digestion)&lt;br&gt; <br /> <br /> [[Image:Cre 2348.jpg]]&lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div>

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