https://eln.excellgen.com/index.php?action=history&feed=atom&title=ELISA_Coating_Buffer 2026-10-18T05:34:20Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=ELISA_Coating_Buffer&diff=785&oldid=prev 2011-05-01T22:34:29Z

<p>Created page with &quot;&#039;&#039;&#039;Bicarbonate/carbonate coating buffer (100 mM)&#039;&#039;&#039;&lt;br&gt; 3.03 g Na2CO3,&lt;br&gt;6.0 g NaHCO3&lt;br&gt;1000 ml distilled water&lt;br&gt;pH 9.6, &lt;br&gt;&#039;&#039;&#039;PBS:&#039;&#039;&#039;&lt;br&gt;1.16 g Na2HPO4,&lt;br&gt;0.1 g KCl,&lt;br...&quot;</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Bicarbonate/carbonate coating buffer (100 mM)&#039;&#039;&#039;&lt;br&gt;<br /> 3.03 g Na2CO3,&lt;br&gt;6.0 g NaHCO3&lt;br&gt;1000 ml distilled water&lt;br&gt;pH 9.6,<br /> <br /> <br /> <br /> &lt;br&gt;&#039;&#039;&#039;PBS:&#039;&#039;&#039;&lt;br&gt;1.16 g Na2HPO4,&lt;br&gt;0.1 g KCl,&lt;br&gt;0.1 g K3PO4,&lt;br&gt;4.0 g NaCl (500 ml distilled water) pH 7.4.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> A protein (antigen or antibody) concentration of 1-10 ug/mL in coating buffer is usually sufficient.&lt;br&gt;<br /> <br /> [[Category:ELISA]][[Category:Buffer_Recipe]]</div>

Paul.xu